Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 1998; 4(6): 536-539
Published online Dec 15, 1998. doi: 10.3748/wjg.v4.i6.536
Preliminary study on the production of transgenic mice harboring hepatitis B virus X gene
Huan-Zhang Zhu, Guo-Xiang Cheng, Jian-Qu Chen, Shu-Yuan Kuang, Yong Cheng, Xin-Li Zhang, Hou-Da Li, Shao-Fu Xu, Jing-Quan Shi, Geng-Sun Qian, Jian-Ren Gu
Huan-Zhang Zhu, Xin-Li Zhang, Jing-Quan Shi, Department of Pathology, Third Military Medical University, Chongqing 400038, China
Guo-Xiang Cheng, Jian-Qu Chen, Yong Cheng, Hou-Da Li, Shao-Fu Xu, Laboratory for Bioengineering, Yangzhou 225009, Jiangsu Province, China
Shu-Yuan Kuang, Geng-Sun Qian, Jian-Ren Gu, Shanghai Cancer Institute, Shanghai 200032, China
Huan-Zhang Zhu, male, born on February 26, 1964 and graduated from Department of Medicine in the Third Military Medical University, lecturer of pathology, having 6 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Guo-Xiang Cheng, Laboratory for Bioengineering, Jiangsu Province, Yangzhou University 225009, Jiansu Province, China
Telephone: +86-514-7979348
Received: June 10, 1998
Revised: August 21, 1998
Accepted: November 14, 1998
Published online: December 15, 1998

AIM: To establish transgenic mice lineage harboring hepatitis B virus X gene and to provide an efficient animal model for studying the exact role of the HBx gene in the process of hepatocarcinogenesis.

METHODS: The HBx transgenic mice were produced by microinjecting the construct with X gene of HBV (subtype adr) DNA fragment into fertilzed eggs derived from inbred C57BL/6 strain; transgenic mice were identified by using Nested PCR; expression and phenotype of HBx gene were analyzed in liver from transgenic mic at the age of 8 weeks by RT-PCR, pathologic examination and periodic acid-schiff staining (PAS), respectively.

RESULTS: Five hundred and fourteen fertilized eggs of C57 BL/6 mice were microinjected with recombinant retroviral DNA fragment, and 368 survival eggs injected were transferred to the oviducts of 18 pseudopregnant recipient mice, 8 of them became pregnant and gave birth to 20 F1 offspring. Of 20 offsprings, four males and two females carried the hybrid gene (HBx gene). Four male mice were determined as founder, named X1, X5, X9 and X15. These founders were back crossed to set up F1 generations with other ibred C57BL/6 mice or transgenic littermates, respectively. Transmission of HBx gene in F1 offspring of X1, X5 and X9 except in X15 followed Mendelian rules. The expression of HBx mRNA was detected in liver of F1 offspring from the founder mice (X1 and X9), which showed vacuolation lesion and glycogen positive foci.

CONCLUSION: Transgenic mice harboring HBx gene were preliminarily established.

Keywords: hepatitis B virus, gene, viral, transgenic animals, liver neoplasms, diseases models, animal