Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 1998; 4(5): 412-414
Published online Oct 15, 1998. doi: 10.3748/wjg.v4.i5.412
Relationship between expression of CD44v6 and nm23-H1 and tumor invasion and metastasis in hepatocellular carcinoma
Cheng-Zhi Xiao, Yi-Min Dai, Hong-Yu Yu, Jian-Jun Wang, Can-Rong Ni
Cheng-Zhi Xiao, Yi-Min Dai, Hong-Yu Yu, Jian-Jun Wang, Can-Rong Ni, Department of Pathology, Second Military Medical University, Shanghai 200433, China
Cheng-Zhi Xiao, male, born on 1970-05-14 in Meihekou City, Jilin Province, graduated from Second Military Medical University in 1998, with a master degree of pathology.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Cheng-Zhi Xiao, Department of Pathology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China
Telephone: +86-21-25070279
Received: July 6, 1998
Revised: August 15, 1998
Accepted: September 21, 1998
Published online: October 15, 1998
Abstract

AIM: To detect the expression of CD44v6 mRNA and nm23-H1 mRNA in hepatocellular carcinoma (HCC) by in situ hybridization, and to evaluate the relationship between their expression and also relationship between their expressions and tumor invasion and metastasis.

METHODS: CD44v6 cDNA probe was synthesized with PCR technique and the nm23-H1 cRNA probe by in vitro transcription. The expression of CD44v6 mRNA and nm23-H1 mRNA was detected by in situ hybridization.

RESULTS: In group with high invasion and metastasis potential, the positive rates of CD44v6 mRNA and nm23-H1 mRNA were 80% (8/10) and 40% (4/10),in group with poor invasion and metastasis potential, they were 21.7% (5/23) and 91.3% (21/23). There was a positive correlation between the expression of CD44v6 mRNA and tumor invasion and metastasis potential in HCC (P < 0.01), and a reverse correlation between the expression of nm23-H1 mRNA and tumor invasion and metastasis potential (P < 0.01) and a reverse correlation in the expression between CD44v6 mRNA and nm23-H1 mRNA in HCC (P < 0.01).

CONCLUSION: Detection of CD44v6 mRNA and nm23-H1 mRNA may be useful for tumor invasion and metastasis in HCC.

Keywords: liver neoplasms; carcinoma, hepatocellular; neoplasm invasion; neoplasm metastasis; CD44v6; nm23-H1; RNA, messenge