Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 15, 1998; 4(3): 210-213
Published online Jun 15, 1998. doi: 10.3748/wjg.v4.i3.210
Transduction of human hepatocellular carcinoma cells with human γ-interferon gene via retroviral vector
Shu-Bing Qian, Shi-Shu Chen
Shu-Bing Qian, Shi-Shu Chen, Department of Biochemistry and Molecular Biology, Research Center for Human Gene Therapy, Shanghai Second Medical University, Shanghai 200025, China
Shu-Bing Qian, male, born on 1970-04-03 in Zhejiang province, Han nationality, graduated from Shanghai Second Medical University as a postgraduate in 1997, lecturer of biochemistry, majoring cancer molecular biology and gene therapy, having 6 papers published. Presented at the First International Symposium on Gene Therapy, Beijing, July 7-10, 1997
Author contributions: All authors contributed equally to the work.
Correspondence to: Shi-Shu Chen, Research Center for Human Gene Chongqing Rd, Shanghai 200025, China. zpwu@fudan.ac.cn
Telephone: +86-21-63846590 ext 400 Fax: +86-21-6384961
Received: October 28, 1997
Revised: March 16, 1998
Accepted: May 15, 1998
Published online: June 15, 1998

AIM: To investigate the therapeutic potential of gamma interferon (IFN-γ) genemodified human hepatocellular carcinoma (HCC) cells.

METHODS: The IFN-γ gene was introduced retrovirally into four HCC cell lines. Secreted IFN-γ activity was assessed using bioassay. The expression of MHC molecules was detected by FACS. Tumorigenicity was analysed by tumor formation in nude mice.

RESULTS: Four IFN-γ gene transduced HCC cell lines secreted different amounts of IFN-γ, as in the same case of five clones derived from one HCC cell line. Transduction with IFN-γ caused significant increase in the expression of major histocompatibility complex (MHC) antigens on HCC cells. The expression of HLA class I was increased by 2-3 times in terms of mean fluorescence intensities, while for class II expression, the percentage of positive cells augmented from < 10% to > 50%. When equal amount of tumor cells were injected into nude mice, the tumor igenicity some transduced cells decreased dramantically.

CONCLUSION: IFN-γ gene transduction can convert weakly imunogenic HCC cells to activate antitumor immune response, and further pave the way for the future use of such gene modified tumor cells as a modality for the cancer immunotherapy.

Keywords: Carcinoma, hepatocellular/therapy, interferon II/genetics, retroviridae, immunotherapy, adoptive