Published online Apr 15, 1998. doi: 10.3748/wjg.v4.i2.117
Revised: January 3, 1998
Accepted: February 5, 1998
Published online: April 15, 1998
AIM: To establish a method of labeling anti-hepatoma McAb (HAb18) Fab fragment modifier with 99mTc.
METHODS: HAb18 Fab was modified with 2-iminotholane and labeled with 99mTc by transchelation from 99mTc GH. Labeling yield, radiochemical purity and immunoreactivity were determined by thin layer chromatography (TLC-SG), paper chromatography (PC), gel chromatography (GC) and cell binding assay, respectively. The nude mice bearing human hepatoma were used for radioimmunoimaging (RII).
RESULTS: A radiolabeling yield of 50%-80% was obtained, and immunoreactivity (IR) was 30%-40%. Radioimaging results showed that 99mTc-HAb18 McAb Fab fragment was concentrated in the tumor 4-8 h after injection, and the maximum concentration was seen in 12-24 h, and the T/NT value was 5.18 and 7.48 at 6 h and 8 h after the injection.
CONCLUSION: 99mTc-HAb18 McAb Fab fragment could be specifically localized in the tumor of nude mice bearing human hepatocellular carcinoma within 24 h and this method might be effectively used for labeling McAb Fab fragment with 99mTc.