Traditional Medicine
Copyright ©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 15, 1997; 3(1): 50-53
Published online Mar 15, 1997. doi: 10.3748/wjg.v3.i1.50
Co-regulative effects of the cAMP/PKA and DAG/PKC signal pathways on human gastric cancer cells during differentiation induced by traditional Chinese medicines
Shan-Qing Gu, Yun-Yan Liang, Li-Ren Fan, Bao-Yuan Li, Dai-Shu Wang
Shan-Qing Gu, Li-Ren Fan, Lab of Oncology, Qingdao No.6 People’s Hospital, Qingdao 266033, Shandong Province, China
Yun-Yan Liang, Bao-Yuan Li, Dai-Shu Wang, Lab of Cell Biology, Beijing Institute for Cancer Research, Beijing 100034, China
Shan-Qing Gu, male, born in June 1970 in Weifang, Shandong Province, admitted to a postgraduate program of oncology in Beijing Medical University, has 4 papers published
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation of China, No. 938800202.
Correspondence to: Dr. Dai-Shu Wang, Professor, Lab of Oncology, Qingdao No.6 People’s Hospital, Qingdao 266033, Shandong Province, China
Telephone: +86-532-3833866
Received: September 1, 1996
Revised: November 5, 1996
Accepted: December 14, 1996
Published online: March 15, 1997
Abstract

AIM: To evaluate the role of cAMP/PKA and DAG/PKC pathways of MGc80-3 cells treated with a traditional Chinese medicine compound, bailong preparation (bailong).

METHODS: cAMP level, DAG content and activities of PKA and PKC were measured in different groups: control; 1.8 g/L bailong; 1.8 g/L bailong + 20 mg/L PKA inhibitor; and 5 μmol/L PKC inhibitor.

RESULTS: When MGc80-3 cells were treated with bailong for 3 h, cAMP level and PKA activity were 113% and 19.7% higher than those of the control, while DAG content and PKC activity were 47.0% and 64.2% lower than those of the control. When the PKC pathway was blocked by PKC inhibitor GF-109203 X, cAMP level and PKA activity were increased by 78.8% and 33.5% compared to inhibitor GF-109203 X, and cAMP level and PKA activity were increased by 78.8% and 33.5% compared to the control, while the DAG content and PKC activity were decreased by 40.3% and 56.3%. When MGc80-3 cells were treated with bailong and PKA inhibitor blocked PKA pathways at the same time, cAMP level and PKA activity were decreased by 46.0% and 28.9%. On the other hand, DAG content and PKC activity were increased by 50.7% and 51.6% compared to the bailong group.

CONCLUSION: There is a relationship of cause and effect between differentiation of MGc80-3 cells and the signal pathways. The results of this study are similar to that of hexamethylenebisacetamide (HMBA), suggesting that the two signal systems are the foundation of proliferative regulation of MGc80-3 cells treated with Chinese medicine bailong or HMBA.

Keywords: Chinese medicine; cAMP/PKA; DAG/PKC; Gastric cancer