Original Research
Copyright ©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 15, 1997; 3(1): 19-21
Published online Mar 15, 1997. doi: 10.3748/wjg.v3.i1.19
Studies on the relationship between the point mutation of ras oncogenes and the prognosis of patients with gastric cancer
Dian-Chun Fang, Yuan-Hui Luo, Rong Lu, Wei-Wen Liu
Dian-Chun Fang, Yuan-Hui Luo, Rong Lu, Wei-Wen Liu, Department of Gastroentrology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China
Dian-Chun Fang, Professor of internal medicine, with 102 published papers
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Dian-Chun Fang, Professor, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China
Telephone: +86-811-5318301
Received: August 21, 1996
Revised: September 30, 1996
Accepted: January 31, 1997
Published online: March 15, 1997
Abstract

AIM: To study the relationship between the point mutation of ras oncogenes and the prognosis of patients with gastric cancer.

METHODS: The point mutations at codon 12 and 61 of c-Ha-ras, at codon 12 and 13 of K-ras, and at codon 12 of N-ras were studied with PCR-RFLP in 88 formalin fixed and paraffin embedded specimens of gastric cancer.

RESULTS: It was found that the overall rate of point mutation of ras oncogenes was 18.2% and the positivity of the point mutation of ras oncogenes was related to the cancerous invasion of the serosa, the status of lymph node metastasis, the stage of cancer and the survival time after surgery.

CONCLUSION: The findings suggest that the determination of point mutations of ras oncogenes can be used to determine the prognosis of patients with gastric cancer.

Keywords: Stomach neoplasms; Genes; Ras point mutation; Polymerase chain reaction; Polymorphism: Restriction fragment length; Prognosis