Published online Apr 21, 2018. doi: 10.3748/wjg.v24.i15.1622
Peer-review started: February 14, 2018
First decision: March 9, 2018
Revised: March 16, 2018
Accepted: March 25, 2018
Article in press: March 25,2018
Published online: April 21, 2018
To investigate whether the liver resection volume in a newly developed nonalcoholic steatohepatitis (NASH) model influences surgical outcome.
For establishment of a NASH model, mice were fed a high-fat diet for 4 wk, administered CCl4 for the last 2 wk, and administered T0901317 for the last 5 d. We divided these mice into two groups: A 30% partial hepatectomy (PH) of NASH liver group and a 70% PH of NASH liver group. In addition, a 70% PH of normal liver group served as the control. Each group was evaluated for survival rate, regeneration, apoptosis, necrosis and DNA expression after PH.
In the 70% PH of NASH group, the survival rate was significantly decreased compared with that in the control and 30% PH of NASH groups (P < 0.01). 10 of 32 mice in the NASH 70% PH group died within 48 h after PH. Serum aspartate aminotransferase (AST) levels and total bilirubin (T-Bil) in the NASH 70% PH group were significantly higher than the levels in the other two groups (AST: P < 0.05, T-Bil: P < 0.01). In both PH of NASH groups, signaling proteins involved in regeneration were expressed at lower levels than those in the control group (P < 0.01). The 70% PH of NASH group also exhibited a lower number of Ki-67-positive cells and higher rates of apoptosis and necrosis than the NASH 30% PH group (P < 0.01). In addition, DNA microarray assays showed differences in gene expression associated with cell cycle arrest and apoptosis.
The function of the residual liver is impaired in fatty liver compared to normal liver. A larger residual volume is required to maintain liver functions in mice with NASH.
Core tip: We report whether the liver resection volume in the nonalcoholic steatohepatitis (NASH) model influences surgical outcome. The population of patients with NASH has been increasing. However, few animal models fully reflect both the histopathology and pathophysiology of NASH in humans. We established a novel experimental NASH model that exhibited the same characteristics as NASH in humans. This study elucidates the metabolism of the residual liver after a hepatectomy with NASH. Compared with normal liver, the residual NASH liver function is impaired, especially its regenerative ability. Therefore, a larger residual volume is required to maintain liver function in NASH liver after partial hepatectomy.