Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 21, 2017; 23(31): 5680-5691
Published online Aug 21, 2017. doi: 10.3748/wjg.v23.i31.5680
Effects of albumin/glutaraldehyde glue on healing of colonic anastomosis in rats
Kalliopi Despoudi, Ioannis Mantzoros, Orestis Ioannidis, Aggeliki Cheva, Nikolaos Antoniou, Dimitrios Konstantaras, Savvas Symeonidis, Manousos George Pramateftakis, Efstathios Kotidis, Stamatis Angelopoulos, Konstantinos Tsalis
Kalliopi Despoudi, Ioannis Mantzoros, Orestis Ioannidis, Nikolaos Antoniou, Dimitrios Konstantaras, Savvas Symeonidis, Manousos George Pramateftakis, Efstathios Kotidis, Stamatis Angelopoulos, Konstantinos Tsalis, Fourth Surgical Department, Medical School, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece
Aggeliki Cheva, Department of Pathology, General Hospital “G. Papanikolaou”, 57010 Thessaloniki, Greece
Author contributions: Despoudi K, Mantzoros I and Ioannidis O contributed equally to this work; Despoudi K, Pramateftakis MG, Angelopoulos S and Tsalis K designed coordinated the research; Despoudi K, Mantzoros I, Antoniou N, Konstantaras D and Symeonidis S performed the experiments; Despoudi K, Antoniou N, Konstantaras D, Pramateftakis MG and Kotidis E participated equally in treatment of animals; Despoudi K, Ioannidis O and Cheva A analysed the data; Despoudi K, Mantzoros I, Ioannidis O and Cheva A wrote the paper; Despoudi K, Mantzoros I, Ioannidis O, Cheva A, Antoniou N, Konstantaras D, Pramateftakis MG, Kotidis E, Angelopoulos S and Tsalis K significantly contributed to the linguistic formatting and correction of the manuscript, revised it critically for important intellectual content, and were responsible for final proof reading of the article.
Institutional review board statement: The study was reviewed and approved by the Faculty of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece Institutional Review Board.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Veterinary Manager of the Prefecture of Thessaloniki [IACUC protocol number: (S.N.: 13/11872/11-09-08)].
Conflict-of-interest statement: No conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Orestis Ioannidis, MD, MSc, PhD, Surgeon, Scientific Fellow, Fourth Surgical Department, Medical School, Aristotle University of Thessaloniki, Alexandrou Mihailidi 13, 54640 Thessaloniki, Greece. telonakos@hotmail.com
Telephone: +30-231-0814161 Fax: +30-231-0551301
Received: December 29, 2016
Peer-review started: December 30, 2016
First decision: February 13, 2017
Revised: June 8, 2017
Accepted: July 22, 2017
Article in press: July 24, 2017
Published online: August 21, 2017
Abstract
AIM

To evaluate the effect of local surgical adhesive glue (albumin/glutaraldehyde-Bioglue) on the healing of colonic anastomoses in rats.

METHODS

Forty Albino-Wistar male rats were randomly divided into two groups, with two subgroups of ten animals each. In the control group, an end-to-end colonic anastomosis was performed after segmental resection. In the Bioglue group, the anastomosis was protected with extraluminar application of adhesive glue containing albumin and glutaraldehyde. Half of the rats were sacrificed on the fourth and the rest on the eighth postoperative day. Anastomoses were resected and macroscopically examined. Bursting pressures were calculated and histological features were graded. Other parameters of healing, such as hydroxyproline and collagenase concentrations, were evaluated. The experimental data were summarized and computed from the results of a one-way ANOVA. Fisher’s exact test was applied to compare percentages.

RESULTS

Bursting pressures, adhesion formation, inflammatory cell infiltration, and collagen deposition were significantly higher on the fourth postoperative day in the albumin/glutaraldehyde group than in the control group. Furthermore, albumin/glutaraldehyde significantly increased adhesion formation, inflammatory cell infiltration, neoangiogenesis, and collagen deposition on the eighth postoperative day. There was no difference in fibroblast activity or hydroxyproline and collagenase concentrations.

CONCLUSION

Albumin/glutaraldehyde, when applied on colonic anastomoses, promotes their healing in rats. Therefore, the application of protective local agents in colonic anastomoses leads to better outcomes.

Keywords: Adhesions, Bursting pressure, Bioglue™, Albumin/glutaraldehyde, Collagen, Colonic anastomosis

Core tip: The present study was designed to investigate the effect of local surgical adhesive glue composed of albumin/glutaraldehyde in the healing of colonic anastomoses in rats. The application of adhesive glue promotes the healing of colonic anastomoses in rats as it significantly increases the bursting pressure in the early period, but it also causes more adhesions and an enhanced inflammatory reaction.