Published online Dec 21, 2016. doi: 10.3748/wjg.v22.i47.10364
Peer-review started: July 8, 2016
First decision: August 8, 2016
Revised: August 25, 2016
Accepted: September 14, 2016
Article in press: September 14, 2016
Published online: December 21, 2016
To explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer.
Western blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells.
Compared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells.
ANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer.
Core tip: Expression of angiopoietin-like protein 2 (ANGPTL2) was significantly elevated in gastric cancer tissues and cells. The higher the level of malignancy of the cancer, the higher the expression of ANGPTL2 became. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays estimated that the proliferative and invasive ability of lentivirus-infected gastric cancer cells were evidently improved compared to the Lv-NC and blank control groups, which indicate that ANGPTL2 was conducive to the proliferation and invasion of gastric cancer cells.