Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 7, 2016; 22(37): 8283-8293
Published online Oct 7, 2016. doi: 10.3748/wjg.v22.i37.8283
Recent advances in mass spectrometry-based proteomics of gastric cancer
Changwon Kang, Yejin Lee, J Eugene Lee
Changwon Kang, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea
Yejin Lee, J Eugene Lee, Center for Bioanalysis, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea
Author contributions: Kang C and Lee JE designed the study and wrote the manuscript; and Lee Y searched the literature and outlined the manuscript.
Supported by the National Research Council of Science and Technology, No. DRC-14-2-KRISS; and the National Research Foundation of Korea, No. 2013056334, No. 2014044403 and No. 2015052849.
Conflict-of-interest statement: No conflicts of interest.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: J Eugene Lee, PhD, Center for Bioanalysis, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea.
Telephone: +82-42-8685114 Fax: +82-42-8685252
Received: April 28, 2016
Peer-review started: May 1, 2016
First decision: June 20, 2016
Revised: July 28, 2016
Accepted: August 10, 2016
Article in press: August 10, 2016
Published online: October 7, 2016

The last decade has witnessed remarkable technological advances in mass spectrometry-based proteomics. The development of proteomics techniques has enabled the reliable analysis of complex proteomes, leading to the identification and quantification of thousands of proteins in gastric cancer cells, tissues, and sera. This quantitative information has been used to profile the anomalies in gastric cancer and provide insights into the pathogenic mechanism of the disease. In this review, we mainly focus on the advances in mass spectrometry and quantitative proteomics that were achieved in the last five years and how these up-and-coming technologies are employed to track biochemical changes in gastric cancer cells. We conclude by presenting a perspective on quantitative proteomics and its future applications in the clinic and translational gastric cancer research.

Keywords: Gastric cancer, Mass spectrometry, Protein identification, Proteomics, Protein quantification

Core tip: Protein identification and quantification by mass spectrometry represent powerful techniques for deciphering the mechanisms underlying the biochemical anomalies that cause human diseases. Due to innovations in mass spectrometry and labeling techniques, cellular protein levels can be monitored routinely with great accuracy. This review provides a brief overview of these technological advances and their applications in gastric cancer biology.