Evidence-Based Medicine
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 21, 2015; 21(7): 2199-2205
Published online Feb 21, 2015. doi: 10.3748/wjg.v21.i7.2199
New cancer suppressor gene for colorectal adenocarcinoma: Filamin A
Zi-Qiang Tian, Jian-Wei Shi, Xiao-Ran Wang, Zhong Li, Gui-Ying Wang
Zi-Qiang Tian, Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China
Jian-Wei Shi, Zhong Li, Department of Surgical Oncology, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
Xiao-Ran Wang, Gui-Ying Wang, Department of the Second General Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China
Author contributions: Wang GY and Tian ZQ designed the research; Li Z performed the research; Shi JW contributed to analytic tools and scientific discussions; Tian ZQ, Shi JW and Wang XR analyzed the data and wrote the paper.
Supported by Hebei Province Health Department of China, No. 14277710D and No. GL2012050.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Gui-Ying Wang, MD, PhD, Professor, Department of the Second General Surgery, Fourth Hospital of Hebei Medical University, No. 12 Jiankang Street, Shijiazhuang 050011, Hebei Province, China. tizq12@vip.163.com
Telephone: +86-0319-86095679 Fax: 86-0319-86032788
Received: May 4, 2014
Peer-review started: May 4, 2014
First decision: May 14, 2014
Revised: May 25, 2014
Accepted: June 12, 2014
Article in press: June 13, 2014
Published online: February 21, 2015
Abstract

AIM: To determine the expression and significance of filamin A (FLNa) in colorectal adenocarcinoma tissue.

METHODS: The expression of FLNa in 46 colorectal cancer tissues and normal tissues was detected by immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, and its relationship with clinical parameters and prognosis was analyzed.

RESULTS: The positive expression of FLNa in cancer tissues was lower than that in normal mucosa, and the difference was statistically significant. The expression of FLNa correlated with liver metastasis, lymph node metastasis and rectal invasion depth, regardless of sex, age, tumor location, tumor size, gross shape and histological type of colorectal carcinoma. Multivariate analysis showed that FLNa was an independent risk factor for postoperative survival of patients with colorectal adenocarcinoma. Moreover, survival analysis showed that the expression level of FLNa was closely related with survival of patients with colorectal adenocarcinoma. The results of RT-PCR and Western blotting were consistent with those of immunohistochemistry.

CONCLUSION: FLNa showed low expression in colorectal adenocarcinoma, high correlation with the incidence and development of colorectal cancer, and was considered an indicator of prognosis.

Keywords: Colorectal carcinoma, Filamin A, Immunohistochemistry, Reverse transcription-polymerase chain reaction, Western blot

Core tip: The expression of filamin A (FLNa) was correlated with liver metastasis, lymph node metastasis and rectal invasion depth, regardless of sex, age, tumor location, tumor size, gross shape and histological type of colorectal carcinoma. FLNa was an independent risk factor for postoperative survival of patients with colorectal adenocarcinoma. FLNa showed low expression in colorectal adenocarcinoma, high correlation with the incidence and development of colorectal cancer, and was considered an indicator of prognosis.