Published online May 28, 2014. doi: 10.3748/wjg.v20.i20.6302
Revised: December 31, 2013
Accepted: January 20, 2014
Published online: May 28, 2014
AIM: To assess the distribution of human leukocyte antigen (HLA)-DQ2 and -DQ8 in Iranian celiac disease (CD) patients and compare them to healthy Iranian controls.
METHODS: To predict the HLA-DQA1 and -DQB1 genes, we used six previously reported HLA-tagging single nucleotide polymorphism to determine HLA genotypes in 59 Iranian patients with ‘biopsy-confirmed’ CD and in 151 healthy Iranian individuals. To test the transferability of the method, 50 cases and controls were also typed using a commercial kit that identifies individual carriers of DQ2, DQ7 and DQ8 alleles.
RESULTS: In this pilot study 97% of CD cases (n = 57) and 58% of controls (n = 87) were carriers of HLA-DQ2 and/or HLA-DQ8 heterodimers, either in the homozygous or heterozygous state. The HLA-DQ pattern of these 57 CD patients: heterozygous DQ2.2 (n = 14) and homozygous DQ2.2 (n = 1), heterozygous DQ2.5 (n = 33) and homozygous DQ2.5 (n = 8), heterozygous DQ8 (n = 13) and homozygous DQ8 (n = 2). Two CD patients were negative for both DQ2 and DQ8 (3%).
CONCLUSION: The prevalence of DQ8 in our CD population was higher than that reported in other populations (25.4%). As reported in other populations, our results underline the primary importance of HLA-DQ alleles in the Iranian population’s susceptibility to CD.
Core tip: We describe, for the first time, the distribution of human leukocyte antigen (HLA)-DQ2/DQ8 alleles in Iranian celiac disease patients compared to healthy Iranian controls. To assess this distribution we applied a single nucleotide polymorphism-based approach, which was developed in European (Caucasian) study samples. We also demonstrate the transferability of such an approach to the Iranian population. Our results underline the primary importance of HLA-DQ alleles in the Iranian population’s susceptibility to celiac disease.