Published online Dec 15, 1996. doi: 10.3748/wjg.v2.i4.238
Revised: September 18, 1996
Accepted: October 9, 1996
Published online: December 15, 1996
AIM: To study the three-dimensional structure and distribution of the lymphatics in the rabbit appendix, and to reveal the correlation between the perifollicular lymphatic sinus (PLS) and lymphatics.
METHODS: Freeze-fractured tissues and lymphatic corrosion cast with the Mercox were used for scanning electron microscopy (SEM), and histologic and semithin sections were used for light microscopy. The Mercox was diluted and injected intraparenchymally into the appendix wall. The injected appendixes were cut and put in a concentrated NaOH solution until the tissues were corroded away.
RESULTS: The lymphatic capillary networks were found in the superficial layer of the mucosa and the lymphatic capillary plexuses were observed in the deep layer of the mucosa. From the plexuses, the short grove-like lymphatic capillaries were connected with the PLS. The luminal side of the sinus looked like a flower basket. Short lymphatic capillaries arising from the bottom of the PLS were continuous with the lymphatics of the submucosa. The lymphatics of the submucosa were connected with the lymphatics running in the muscular layer, then they were led into the serosal lymphatics and drained into the lymphatics in the mesoappendix.
CONCLUSION: The PLS and rich lymphatics in the rabbit appendix may play an important role in the drainage of lymph and the immune function.