Zhang XH, Li XT, Ji XJ, Zhu YJ. Preliminary study on mechanism of drug resistance in human colon cancer cell line HCTV2000. World J Gastroenterol 1996; 2(4): 232-235 [DOI: 10.3748/wjg.v2.i4.232]
Corresponding Author of This Article
Dr. Xue-Tang Li, Department of Pharmacology, Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China
Article-Type of This Article
Clinical Articles
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World J Gastroenterol. Dec 15, 1996; 2(4): 232-235 Published online Dec 15, 1996. doi: 10.3748/wjg.v2.i4.232
Preliminary study on mechanism of drug resistance in human colon cancer cell line HCTV2000
Xiao-Hong Zhang, Xue-Tang Li, Xin-Juan Ji, Ya-Jun Zhu
Xiao-Hong Zhang, Xin-Juan Ji, Ya-Jun Zhu, Department of Pharmacology, Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China
Xue-Tang Li, Department of Pharmacology (Division I), Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China
Xiao-Hong Zhang, Graduated from the Faculty of Virology, Wuhan University in 1989, MS. in Pharmacology from Institute of Materia Medica, Chinese Academy of Medical Science, Peking Union Medical College in 1992; assistant research fellow of Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College; having 5 papers published.
Author contributions: All authors contributed equally to the work.
Supported by the Key Project of the 8th Five-Year Plan of China, No. 85-914-03-10
Correspondence to: Dr. Xue-Tang Li, Department of Pharmacology, Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China
Telephone: +86-10-67780094
Received: July 31, 1996 Revised: August 26, 1996 Accepted: September 18, 1996 Published online: December 15, 1996
Abstract
AIM: To investigate the mechanisms of multidrug resistance in human colon cancer cell line HCTV2000.
METHODS: P-glycoprotein (P-GP) was detected by immunocytochemical staining. Expression and amplification of mdr1 gene were detected by nucleic acid hybridization. Cytotoxicity was measured by MTT method.
RESULTS: HCTV2000 cells showed a positive response to monoclonal antibodies against P-GP encoded by the mdr1 gene. Overexpression of mdr1 mRNA was found, but no evidence of mdr1 gene amplification or rearrangement was suggested. Verapamil increased the accumulation of 3H-VCR inside the cells and partially reversed drug resistance to VCR.
CONCLUSION: Overexpression of mdr1 mRNA is one of the important mechanisms of drug resistance in HCTV2000 cell line; however, there may be some other mechanisms which are also involved in this.
