p53 gene mutations in primary gastric cancer

doi:10.3748/wjg.v2.i1.41

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Clinical Articles

World J Gastroenterol. Mar 25, 1996; 2(1): 41-43
Published online Mar 25, 1996. doi: 10.3748/wjg.v2.i1.41

p53 gene mutations in primary gastric cancer

Zhong-Xin Li, Pin-Yi Liu, Wen-Xin Xu, Bin Cong, Zhi-Xue Ma, Yong Li

Zhong-Xin Li, Master of Medicine having 10 papers published and working in surgery and the molecular biological study of gastric cancer for the past 6 years, Department of Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China

Pin-Yi Liu, Zhi-Xue Ma, Yong Li, Department of Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China

Wen-Xin Xu, Bin Cong, the Molecular Biology Laboratory of Hebei Medical University, Shijuazhuang 050017, Hebei Province, China

Author contributions: All authors contributed equally to the work.

Received: November 12, 1995
Revised: November 30, 1995
Accepted: December 19, 1995
Published online: March 25, 1996

Abstract

AIM: To analyze the relationshio between p53 gene mutations and these parameters including DNA ploidy in Chinese primary gastric cancers.

METHODS: Mutations of the p53 gene in exon5-8 were examined in 20 cases of priary gasric cancer by PCR-SSCP (Polymerase Chain Reaction-Single Strand Conformation Polymorphism) analysis

RESULTS: Mutations were detected in 8 (40%) cases: 2 cases in exon5-6, 2 cases in exon7, 4 cases in exon8. These mutations were detected from stage 0 to stage III. No significant association was found between p53 gene mutations and the clinicopathological parameters such as macroscopic classification, degree of histological differentiation, depth of tumour invasion and lymphonod metastasis. In addition, 66.7% (6 of 9) of aneuploidy tumours had p53 mutations and only 18.2% (2 of 11) of diploid tumours had mutations.

CONCLUSION: These results suggest that p53 gene mutations are related to DNA ploidy alterations and that p53 gene is one of the important tumour suppressor genes in human gastric cancer.

Keywords: Genes, p53, Stomach neoplasms, Mutation, Polymerase chain reaction