Published online Mar 25, 1996. doi: 10.3748/wjg.v2.i1.41
Revised: November 30, 1995
Accepted: December 19, 1995
Published online: March 25, 1996
AIM: To analyze the relationshio between p53 gene mutations and these parameters including DNA ploidy in Chinese primary gastric cancers.
METHODS: Mutations of the p53 gene in exon5-8 were examined in 20 cases of priary gasric cancer by PCR-SSCP (Polymerase Chain Reaction-Single Strand Conformation Polymorphism) analysis
RESULTS: Mutations were detected in 8 (40%) cases: 2 cases in exon5-6, 2 cases in exon7, 4 cases in exon8. These mutations were detected from stage 0 to stage III. No significant association was found between p53 gene mutations and the clinicopathological parameters such as macroscopic classification, degree of histological differentiation, depth of tumour invasion and lymphonod metastasis. In addition, 66.7% (6 of 9) of aneuploidy tumours had p53 mutations and only 18.2% (2 of 11) of diploid tumours had mutations.
CONCLUSION: These results suggest that p53 gene mutations are related to DNA ploidy alterations and that p53 gene is one of the important tumour suppressor genes in human gastric cancer.