Published online Dec 14, 2013. doi: 10.3748/wjg.v19.i46.8758
Revised: October 9, 2013
Accepted: October 19, 2013
Published online: December 14, 2013
AIM: To investigate the correlation between the Helicobacter pylori (H. pylori) infection and host genetic background of healthy populations in Indonesia.
METHODS: In March 2007, epidemiological studies were undertaken on the general population of a city in Indonesia (Mataram, Lombok). The participants included 107 men and 187 women, whose ages ranged from 6 to 74 years old, with an average age of 34.0 (± 14.4) (± SD). The H. pylori of subject by UBT method determination, and through the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) method parsing the single nucleotide polymorphism of interleukin (IL)-8, IL-4, IL-1β, CD14, tumor necrosis factor (TNF-α) and tyrosine-protein phosphates non-receptor type 11 (PTPN11) genotypes. The experimental data were analyzed by the statistical software SAS.
RESULTS: The H. pylori infection rates in the healthy Indonesian population studied were 8.4% for men and 12.8% for women; no obvious differences were noted for H. pylori infection rates by sex or age. TC genotypes of IL-4, TC and CC genotypes of TNF-α, and GA genotypes of PTPN11, were higher in frequency. Both CC and TC genotype of TNF-α T-1031C loci featured higher expressions in the healthy Indonesian population Indonesia studied of (OR = 1.99; 95%CI: 0.67-5.89) and (OR = 1.66; 95%CI: 0.73-3.76), respectively. C allele of IL-1β T-31C gene locus was at a higher risk (OR = 1.11; 95%CI: 0.70-1.73) of H. pylori infection, but no statistical significance was found in our study.
CONCLUSION: We reveal that the association between the TNF-α and IL-1β genotypes may be the susceptibility of H. pylori in the studied population.
Core tip: We found single nucleotide polymorphism of tumor necrosis factor-α and allele of interleukin-1β having high frequency in the healthy Indonesian population, which may be associated with potential contact with Helicobacter pylori (H. pylori) infection. Throughout, H. pylori studies were conducted in patients, and treatment was based on quadruple antibiotics to eradicate H. pylori infection in clinical trials. However, the implications of the individual differences in recurrent infections and drug resistance of H. pylori and other issues must be addressed. Therefore, vaccine development for prevention of H. pylori will be a topical issue in the coming years.