Original Article
Copyright ©2012 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Dec 28, 2012; 18(48): 7251-7261
Published online Dec 28, 2012. doi: 10.3748/wjg.v18.i48.7251
Single nucleotide polymorphisms in the CDH17 gene of colorectal carcinoma
Ren-Yin Chen, Juan-Juan Cao, Juan Chen, Jian-Ping Yang, Xiao-Bo Liu, Guo-Qiang Zhao, Yu-Feng Zhang
Ren-Yin Chen, Juan-Juan Cao, Jian-Ping Yang, Xiao-Bo Liu, Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
Ren-Yin Chen, Juan-Juan Cao, Jian-Ping Yang, Xiao-Bo Liu, Henan Key Laboratory for Tumor Pathology, Zhengzhou 450052, Henan Province, China
Juan Chen, Zhengzhou University School of Pharmaceutical Sciences, Zhengzhou 450052, Henan Province, China
Guo-Qiang Zhao, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan Province, China
Yu-Feng Zhang, Department of Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
Author contributions: Chen RY designed and examined the experiment and wrote the paper; Cao JJ and Chen J performed the experiment; Yang JP collected pathological information and checked the pathological diagnosis; Liu XB collected samples; Zhao GQ designed the polymerase chain reaction primer; and Zhang YF designed, directed and examined the experiment.
Supported by 2010A310011 Henan Provincial Department of Education on Natural Science Research Projects
Correspondence to: Yu-Feng Zhang, Professor, Archiater, Department of Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China. zhangyufeng@zzu.edu.cn
Telephone: +86-371-66862181 Fax: +86-371-66964927
Received: August 23, 2012
Revised: October 31, 2012
Accepted: November 11, 2012
Published online: December 28, 2012
Abstract

AIM: To investigate the relationship between c.343A>G and c.2216A>C polymorphism sites in the CDH17 gene and colorectal carcinoma.

METHODS: Ninety-three non-consanguineous colorectal carcinoma patients admitted to the Department of Oncology at the First Affiliated Hospital of Zhengzhou University were included in this study. Ninety-three peripheral venous blood samples, of approximately one milliliter from each patient, were collected between December 2009 and August 2010. The genomic DNA of these peripheral venous blood samples were extracted and purified using a Fermentas Genomic DNA Purification Kit (Fermentas, CA) according to the manufacturer’s protocol. The single nucleotide polymorphisms (SNPs) of the liver-intestine cadherin (CDH17) gene c.343A>G and c.2216A>C were determined by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP) in 93 peripheral venous blood samples from patients suffering with colorectal carcinoma. Typical samples that showed different migration bands in SSCP were confirmed by sequencing. Directed DNA sequencing was used to check the correctness of the genotype results from the PCR-SSCP method.

RESULTS: There was a significant association between the c.2216 A>C SNPs of the CDH17 gene and the tumor-node-metastasis (TNM) grade, as well as with lymph node status, in 93 peripheral venous blood samples from colorectal carcinoma patients. The genotype frequencies of A/C, A/A, and C/C were 12.90%, 33.33% and 53.76%, respectively. There was a significant correlation between lymph node metastasis, TNM grade, and the genotype distribution (P < 0.05). The C/C genotype raised the risk of lymph node metastasis and the TNM grade. There was a significant difference in the TNM grade and lymph node metastasis between the A/A and C/C genotypes (P = 0.003 and P = 0.013, respectively). Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis and have a higher TNM grade. The difference between the TNM grades, as well as the lymph node metastasis of the two alleles, was statistically significant (P < 0.01).

CONCLUSION: The SNPs of the CDH17 gene c.2216 A>C might be clinically important in the prognosis of colorectal carcinoma.

Keywords: Single nucleotide polymorphisms, Liver-intestine cadherin, Colorectal carcinoma, Polymerase chain reaction-single strand conformation polymorphism method