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Effect of biologically active fraction of Nardostachys jatamansi on cerulein-induced acute pancreatitis
Gi-Sang Bae, Min-Sun Kim, Kyoung-Chel Park, Bon Soon Koo, Il-Joo Jo, Sun Bok Choi, Dong-Sung Lee, Youn-Chul Kim, Tae-Hyeon Kim, Sang-Wan Seo, Yong Kook Shin, Ho-Joon Song, Sung-Joo Park
Gi-Sang Bae, Min-Sun Kim, Kyoung-Chel Park, Bon Soon Koo, Il-Joo Jo, Sun Bok Choi, Ho-Joon Song, Sung-Joo Park, Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea
Il-Joo Jo, Department of Beauty Science, Kwangju Women’s University, Kwangju 506-713, South Korea
Dong-Sung Lee, Youn-Chul Kim, Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan 570-749, South Korea
Tae-Hyeon Kim, Department of Internal Medicine, College of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea
Sang-Wan Seo, Yong Kook Shin, ChungBuk Technopark Bio Center, Jecheon, ChungBuk 390-250, South Korea
Author contributions: Bae GS and Kim MS contributed equally to the research; Bae GS and Park SJ designed the research; Bae GS, Kim MS, Park KC, Koo BS, Jo IJ, Choi SB, Lee DS, Kim YC, Kim TH, Seo SW, Shin YK and Song HJ performed the research; Bae GS and Park SJ analyzed the data; Bae GS and Park SJ wrote the paper.
Supported by The Ministry of Education, Science and Technology at Wonkwang University, No. MEST 2010-0017094
Correspondence to: Sung-Joo Park, PhD, MD, Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea. parksj08@wku.ac.kr
Telephone: +82-63-8506450 Fax: +82-63-8562283
Received: September 29, 2011
Revised: April 16, 2012
Accepted: May 12, 2012
Published online: July 7, 2012
AIM: To determine if the fraction of Nardostachys jatamansi (NJ) has the potential to ameliorate the severity of acute pancreatitis (AP).
METHODS: Mice were administered the biologically active fraction of NJ, i.e., the 4th fraction (NJ4), intraperitoneally, and then injected with the stable cholecystokinin analogue cerulein hourly for 6 h. Six hours after the last cerulein injection, the pancreas, lung, and blood were harvested for morphological examination, measurement of cytokine expression, and examination of neutrophil infiltration.
RESULTS: NJ4 administration attenuated the severity of AP and lung injury associated with AP. It also reduced cytokine production and neutrophil infiltration and resulted in the in vivo up-regulation of heme oxygenase-1 (HO-1). Furthermore, NJ4 and its biologically active fraction, NJ4-2 inhibited the cerulein-induced death of acinar cells by inducing HO-1 in isolated pancreatic acinar cells.
CONCLUSION: These results suggest that NJ4 may be a candidate fraction offering protection in AP and NJ4 might ameliorate the severity of pancreatitis by inducing HO-1 expression.
