Brief Article
Copyright ©2012 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jun 28, 2012; 18(24): 3129-3137
Published online Jun 28, 2012. doi: 10.3748/wjg.v18.i24.3129
Knockdown of liver-intestine cadherin decreases BGC823 cell invasiveness and metastasis in vivo
Yu Xu, Jin Zhang, Qi-Sheng Liu, Wei-Guo Dong
Yu Xu, Department of Otolaryngology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Jin Zhang, Wei-Guo Dong, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Qi-Sheng Liu, Department of Gastroenterology, Central Hospital of Xianning City, Xianning 437000, Hubei Province, China
Author contributions: Xu Y, Zhang J and Liu QS contributed equally to this work; Xu Y and Dong WG designed the research; Zhang J and Liu QS performed the research and analyzed the data; Zhang J and Xu Y wrote the paper.
Supported by The National Natural Science Foundation of China, No. 30871147
Correspondence to: Wei-Guo Dong, Professor, Department of Gastroenterology, Renmin Hospital of Wuhan University, No. 9 Ziyang Road, Wuhan 430060, Hubei Province, China.
Telephone: +86-27-88041911 Fax: +86-27-88042292
Received: November 11, 2010
Revised: May 6, 2011
Accepted: May 12, 2012
Published online: June 28, 2012

AIM: To assess BGC823 gastric cancer (GC) cell metastasis after knockdown of liver-intestine cadherin (CDH17) and the therapeutic value of CDH17-RNAi-lentivirus in vivo.

METHODS: We evaluated primary tumor growth and assessed local infiltration and systemic tumor dissemination using an orthotopic implantation technique. The therapeutic value of CDH17 knockdown was examined by intratumoral administration of CDH17-RNA interference (RNAi)-lentivirus in an established GC tumor xenograft mouse model. Furthermore, a comparative proteomic approach was utilized to identify differentially expressed proteins in BGC823 and lenti-CDH17-miR-neg cells following CDH17 knockdown.

RESULTS: Metastases in the liver and lung appeared earlier and more frequently in animals with tumors derived from BGC823 or lenti-CDH17-miR-neg cells than in tumors derived from lenti-CDH17-miR-B cells. Average tumor weight and volume in the CDH17-RNAi-lentivirus-treated group were significantly lower than those in the control group (tumor volume: 0.89 ± 0.04 cm3vs 1.16 ± 0.06 cm3, P < 0.05; tumor weight: 1.15 ± 0.58 g vs 2.09 ± 0.08 g, P < 0.05). Fifteen differentially expressed proteins were identified after CDH17 silencing in BGC823 cells, including a variety of cytoskeletal and chaperone proteins as well as proteins involved in metabolism, immunity/defense, cell proliferation and differentiation, cell cycle, and signal transduction.

CONCLUSION: Our data establish a foundation for future studies of the comprehensive protein expression patterns and effects of CDH17 in GC.

Keywords: Cadherin, Gastric cancer, Intratumoral administration, Liver, Orthotopic implantation, Proteomics