Zhou X, Huang SY, Feng JX, Gao YY, Zhao L, Lu J, Huang BQ, Zhang Y. SOX7 is involved in aspirin-mediated growth inhibition of human colorectal cancer cells. World J Gastroenterol 2011; 17(44): 4922-4927 [PMID: 22171135 DOI: 10.3748/wjg.v17.i44.4922]
Corresponding Author of This Article
Dr. Yu Zhang, The Institute of Genetics and Cytology, School of Life Science, Northeast Normal University, Changchun 130024, Jilin Province, China. zhangy288@nenu.edu.cn
Article-Type of This Article
Brief Article
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World J Gastroenterol. Nov 28, 2011; 17(44): 4922-4927 Published online Nov 28, 2011. doi: 10.3748/wjg.v17.i44.4922
SOX7 is involved in aspirin-mediated growth inhibition of human colorectal cancer cells
Xin Zhou, Shu-Yan Huang, Jing-Xin Feng, Yan-Yan Gao, Li Zhao, Jun Lu, Bai-Qu Huang, Yu Zhang
Xin Zhou, Jing-Xin Feng, Yan-Yan Gao, Li Zhao, Jun Lu, Bai-Qu Huang, Yu Zhang, The Institute of Genetics and Cytology, School of Life Science, Northeast Normal University, Changchun 130024, Jilin Province, China
Shu-Yan Huang, Department of Biology, Zhounan High School of Hunan Province, Changsha 410081, Hunan Province, China
Author contributions: Zhou X, Huang SY, Gao YY, Zhao L and Zhang Y designed the research; Zhou X and Huang SY performed the research; Huang SY, Zhou X, Feng JX, Lu J and Huang BQ analyzed the data; Zhou X, Huang SY and Zhang Y wrote the paper; Zhou X and Huang SY contributed equally to the work.
Supported by The National Natural Science Foundation of China, No. 31071149; the Fundamental Research Funds for the Central Universities, No. 09ZDQD01 and No. 10QNJJ014; and the National Science Foundation of China, No. J0830627-2
Correspondence to: Dr. Yu Zhang, The Institute of Genetics and Cytology, School of Life Science, Northeast Normal University, Changchun 130024, Jilin Province, China. zhangy288@nenu.edu.cn
Telephone: +86-431-85099362 Fax: +86-431-85099768
Received: March 22, 2011 Revised: June 21, 2011 Accepted: June 28, 2011 Published online: November 28, 2011
Abstract
AIM: To confirm the role of sex-determining region Y-box 7 (Sox7) in aspirin-mediated growth inhibition of COX-independent human colorectal cancer cells.
METHODS: The cell survival percentage was examined by MTT (Moto-nuclear cell direc cytotoxicity) assay. SOX7 expression was assessed by using reverse transcription-polymerase chain reaction and Western blotting. SB203580 was used to inhibit the p38MAPK signal pathway. SOX7 promoter activity was detected by Luciferase reporter assay.
RESULTS: SOX7 was upregulated by aspirin and was involved in aspirin-mediated growth inhibition of SW480 human colorectal cancer cells. The p38MAPK pathway played a role in aspirin-induced SOX7 expression, during which the AP1 transcription factors c-Jun and c-Fos upregulated SOX7 promoter activities.
RESULTS: SOX7 is upregulated by aspirin and is involved in aspirin-mediated growth inhibition of human colorectal cancer SW480 cells.
