MicroRNA155 is induced in activated CD4+ T cells of TNBS-induced colitis in mice

doi:10.3748/wjg.v16.i7.854

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Feb 21, 2010 (publication date) through Apr 20, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article

World J Gastroenterol. Feb 21, 2010; 16(7): 854-861
Published online Feb 21, 2010. doi: 10.3748/wjg.v16.i7.854

MicroRNA155 is induced in activated CD4⁺ T cells of TNBS-induced colitis in mice

Da-Fan Chen, Bang-Dong Gong, Qing Xie, Qi-Wen Ben, Jun Liu, Yao-Zong Yuan

Da-Fan Chen, Jun Liu, Yao-Zong Yuan, Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Second Road, Shanghai 200025, China

Bang-Dong Gong, Qing Xie, Department of Infectious Disease, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Second Road, Shanghai 200025, China

Qi-Wen Ben, Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai 200433, China

Author contributions: Yuan YZ and Xie Q designed the research; Chen DF and Gong BD performed the majority of experiments; Ben QW and Liu J provided the new reagents and analytic tools and were also involved in editing the manuscript; Yuan YZ, Xie Q and Chen DF analyzed the data; Yuan YZ and Chen DF wrote the paper.

Correspondence to: Yao-Zong Yuan, Professor, Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Second Road, Shanghai 200025, China. yyz28@medmail.com.cn

Telephone: +86-21-64370045 Fax: +86-21-64150773

Received: November 29, 2009
Revised: December 19, 2009
Accepted: December 26, 2009
Published online: February 21, 2010

Abstract

AIM: To investigate the expression of microRNA155 (miRNA155) in trinitrobenzene sulphonic acid (TNBS)-induced colitis and the relationship between miRNA155 and tumor necrosis factor (TNF) expressions.

METHODS: In TNBS colitis mice, miRNA155 and TNF mRNA expressions were measured in colons and CD4⁺ T cells of draining lymph nodes (LNs). CD4⁺ T cells were cultured in vitro with or without anti-CD3/CD28 antibody, and the expressions of miRNA155 and TNF mRNA in cells and TNF concentration in culture media were examined.

RESULTS: miRNA155 and TNF mRNA expressions in colons and in cells of LNs were significantly increased in TNBS colitis compared with controls. In TNBS colitis, miRNA155 and TNF mRNA expressions in CD4⁺ T cells of LNs and TNF concentration in CD4⁺ T cells culture media increased compared with controls. When cultured with anti-CD3/CD28 antibody, miRNA155 and TNF mRNA expressions in CD4⁺ T cells and TNF concentration in the CD4⁺ T cells culture media were significantly higher than those cultured without anti-CD3/CD28 antibody. Following analysis using the Pearson’s correlation coefficient, miRNA155 expression had a significant positive correlation with either TNF mRNA expression in CD4⁺ T cells (r = 0.860, P < 0.05) or TNF concentration in CD4⁺ T cells culture media (r = 0.892, P < 0.05).

CONCLUSION: miRNA155 is induced in colons and activated CD4⁺ T cells in TNBS colitis, and the levels of miRNA155 and TNF expressions have a significant positive correlation.

Keywords: Colitis, Crohn’s disease, Lymph nodes, microRNA, Tumor necrosis factor