Cui XN, Tang JW, Song B, Wang B, Chen SY, Hou L. High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells. World J Gastroenterol 2009; 15(48): 6117-6122 [PMID: 20027687 DOI: 10.3748/wjg.15.6117]
Corresponding Author of This Article
Li Hou, Professor, Department of Pathology, Dalian Medical University, Dalian 116044, Liaoning Province, China. lihou1972@hotmail.com
Article-Type of This Article
Brief Article
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World J Gastroenterol. Dec 28, 2009; 15(48): 6117-6122 Published online Dec 28, 2009. doi: 10.3748/wjg.15.6117
High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells
Xiao-Nan Cui, Jian-Wu Tang, Bo Song, Bo Wang, Shan-Yan Chen, Li Hou
Xiao-Nan Cui, Department of Oncology, the 1st Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
Jian-Wu Tang, Bo Song, Bo Wang, Li Hou, Department of Pathology, Dalian Medical University, Dalian 116044, Liaoning Province, China
Shan-Yan Chen, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430074, Hubei Province, China
Author contributions: Cui XN, Hou L, Tang JW, Song B, Wang B and Chen SY performed the in vitro experiments; Song B, Wang B and Chen SY provided analytical tools; Cui XN and Hou L designed the study and wrote the manuscript.
Supported by National Natural Science Foundation of China, No. 30500586
Correspondence to: Li Hou, Professor, Department of Pathology, Dalian Medical University, Dalian 116044, Liaoning Province, China. lihou1972@hotmail.com
Telephone: +86-411-86110322 Fax: +86-411-86110322
Received: September 26, 2009 Revised: November 16, 2009 Accepted: November 23, 2009 Published online: December 28, 2009
Abstract
AIM: To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells.
METHODS: A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis.
RESULTS: High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo.
CONCLUSION: High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.
