Brief Article
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Oct 21, 2009; 15(39): 4958-4961
Published online Oct 21, 2009. doi: 10.3748/wjg.15.4958
A new polymorphism in the GRP78 is not associated with HBV invasion
Xiao Zhu, Yi Wang, Tao Tao, Dong-Pei Li, Fei-Fei Lan, Wei Zhu, Dan Xie, Hsiang-Fu Kung
Xiao Zhu, Institute of Oncology, Affiliated Tumor Hospital of Guangzhou Medical College, Guangzhou 510095, Guangdong Province, China
Xiao Zhu, Yi Wang, Wei Zhu, Dan Xie, Hsiang-Fu Kung, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, China
Tao Tao, Department of Digestive Diseases, Zibo Central Hospital, Zibo 255000, Shandong Province, China
Dong-Pei Li, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
Fei-Fei Lan, Laboratory of Integrated Biosciences, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong Province, China
Author contributions: Zhu X performed the research and analyzed the data; Wang Y performed parts of the research and analyzed the data; Tao T coordinated the inclusion of subjects; Li DP and Zhu W wrote the manuscript; Lan FF analyzed the data; Xie D and Kung HF designed the research.
Supported by The grant from Ministry of Science and Technology of China, No. 2006CB910104; the Foundation of Guangzhou Science and Technology Bureau, the People’s Republic of China, No. 2005Z1-E0131
Correspondence to: Dan Xie, MD, PhD, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, No. 651, Dongfeng Road East, Guangzhou 510060, Guangdong Province, China.
Telephone: +86-20-87343193 Fax: +86-20-87343170
Received: July 27, 2009
Revised: August 28, 2009
Accepted: September 4, 2009
Published online: October 21, 2009

AIM: To examine the association between -86 bp (T > A) in the glucose-regulated protein 78 gene (GRP78) and hepatitis B virus (HBV) invasion.

METHODS: DNA was genotyped for the single-nucleotide polymorphism by polymerase chain reaction followed by sequencing in a sample of 382 unrelated HBV carriers and a total of 350 sex- and age-matched healthy controls. Serological markers for HBV infection were determined by enzyme-linked immunosorbent assay kits or clinical chemistry testing.

RESULTS: The distributions of allelotype and genotype in cases were not significantly different from those in controls. In addition, our findings suggested that neither alanine aminotransferase/hepatitis B e antigen nor HBV-DNA were associated with the allele/genotype variation in HBV infected individuals.

CONCLUSION: -86 bp T > A polymorphism in GRP78 gene is not related to the clinical risk and acute exacerbation of HBV invasion.

Keywords: Acute exacerbation, Glucose-regulated protein 78, Hepatitis B virus, Single-nucleotide polymorphism