Brief Articles
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World J Gastroenterol. Sep 7, 2009; 15(33): 4156-4162
Published online Sep 7, 2009. doi: 10.3748/wjg.15.4156
Effect of early preoperative 5-fluorouracil on the integrity of colonic anastomoses in rats
Leyla Ozel, M Sefa Ozel, Ahmet Burak Toros, Melih Kara, Kemal Sırrı Ozkan, Gurkan Tellioglu, Osman Krand, Meral Koyuturk, Ibrahim Berber
Leyla Ozel, Melih Kara, Gurkan Tellioglu, Osman Krand, Ibrahim Berber, Haydarpasa Numune Education and Research Hospital, Department of General Surgery, Istanbul 34668, Turkey
M Sefa Ozel, Haydarpasa Numune Education and Research Hospital, Department of Orthopedics and Traumatology, Istanbul 34668, Turkey
Ahmet Burak Toros, Istanbul Education and Research Hospital, Department of Gastroenterology, Samatya-Istanbul 34310, Turkey
Kemal Sırrı Ozkan, Acibadem Hospital, Department of General Surgery, Istanbul 34718, Turkey
Meral Koyuturk, Istanbul Bilim University, Faculty of Medicine, Department of Histology and Embryology, Istanbul 34394, Turkey
Author contributions: Ozel L and Ozel MS designed the research; Ozel L, Ozel MS and Kara M performed the majority of experiments; Toros AB, Tellioglu G, Krand O and Berber I analyzed the data; Koyuturk M performed histological analysis; Ozel L, Ozel MS and Ozkan KS wrote the paper.
Correspondence to: Dr. Leyla Ozel, Haydarpaşa Numune Education and Research Hospital, Deparment of General Surgery, Istanbul 34668, Turkey.
Telephone: +90-216-4144502 Fax: +90-216-3360565
Received: April 15, 2009
Revised: July 28, 2009
Accepted: August 4, 2009
Published online: September 7, 2009

AIM: To determine the effect of chemotherapy on wound healing by giving early preoperative 5-fluorouracil (5-FU) to rats with colonic anastomoses.

METHODS: Sixty Albino-Wistar male rats (median weight, 235 g) were used in this study. The rats were fed with standard laboratory food and given tap water ad libitum. The animals were divided into three groups: Group 1: Control group (chemotherapy was not administered), Group 2: Intraperitoneally (IP) administered 5-FU group (chemotherapy was administered IP to animals at a dose of 20 mg/kg daily during the 5 d preceeding surgery), Group 3: Intravenously (IV) administered 5-FU group. Chemotherapy was administered via the penil vein, using the same dosing scheme and duration as the second group. After a 3-d rest to minimize the side effects of chemotherapy, both groups underwent surgery. One centimeter of colon was resected 2 cm proximally from the peritoneal reflection, then sutured intermittently and subsequently end-to-end anastomosed. In each group, half the animals were given anaesthesia on the 3rd postoperative (PO) day and the other half on the 7th PO day, for in vivo analytic procedures. The abdominal incisions in the rats were dissected, all the new and old anastomotic segments were clearly seen and bursting pressures of each anastomotic segment, tissue hydroxyproline levels and DNA content were determined to assess the histologic tissue repair process.

RESULTS: When the IV group was compared with the IP group, bursting pressures of the anastomotic segments on the 3rd and 7th PO days, were found to be significantly decreased, hydroxyproline levels at the anastomotic segment on the 7th PO day were significantly decreased (P < 0.01).

CONCLUSION: In this study, we conclude that early preoperative 5-FU, administered IV, negatively affects wound healing. However, IP administered 5-FU does not negatively affect wound healing.

Keywords: 5-fluorouracil, Neoadjuvant therapy, Rats, Wound healing