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World J Gastroenterol. Apr 14, 2009; 15(14): 1677-1689
Published online Apr 14, 2009. doi: 10.3748/wjg.15.1677
Bile-acid-induced cell injury and protection
Maria J Perez, Oscar Briz
Maria J Perez, Oscar Briz, Research Unit, University Hospital of Salamanca; Laboratory of Experimental Hepatology and Drug Targeting, CIBERehd, University of Salamanca, E.D. 129 37007 Salamanca, Spain
Author contributions: Perez MJ and Briz O contributed equally to this work.
Correspondence to: Maria J Perez, Department of Biochemistry and Molecular Biology Campus Miguel de Unamuno E.D. 129, 37007 Salamanca, Spain. mjperez@usal.es
Telephone: +34-923-294781
Fax: +34-923-294579
Received: November 12, 2008
Revised: January 14, 2009
Accepted: January 21, 2009
Published online: April 14, 2009
Abstract

Several studies have characterized the cellular and molecular mechanisms of hepatocyte injury caused by the retention of hydrophobic bile acids (BAs) in cholestatic diseases. BAs may disrupt cell membranes through their detergent action on lipid components and can promote the generation of reactive oxygen species that, in turn, oxidatively modify lipids, proteins, and nucleic acids, and eventually cause hepatocyte necrosis and apoptosis. Several pathways are involved in triggering hepatocyte apoptosis. Toxic BAs can activate hepatocyte death receptors directly and induce oxidative damage, thereby causing mitochondrial dysfunction, and induce endoplasmic reticulum stress. When these compounds are taken up and accumulate inside biliary cells, they can also cause apoptosis. Regarding extrahepatic tissues, the accumulation of BAs in the systemic circulation may contribute to endothelial injury in the kidney and lungs. In gastrointestinal cells, BAs may behave as cancer promoters through an indirect mechanism involving oxidative stress and DNA damage, as well as acting as selection agents for apoptosis-resistant cells. The accumulation of BAs may have also deleterious effects on placental and fetal cells. However, other BAs, such as ursodeoxycholic acid, have been shown to modulate BA-induced injury in hepatocytes. The major beneficial effects of treatment with ursodeoxycholic acid are protection against cytotoxicity due to more toxic BAs; the stimulation of hepatobiliary secretion; antioxidant activity, due in part to an enhancement in glutathione levels; and the inhibition of liver cell apoptosis. Other natural BAs or their derivatives, such as cholyl-N-methylglycine or cholylsarcosine, have also aroused pharmacological interest owing to their protective properties.

Keywords: Apoptosis; Cholestasis; Liver; Necrosis; Oxidative stress; Ursodeoxycholic acid