Antisense expression of PKC&alpha; improved sensitivity of SGC7901/VCR cells to doxorubicin

doi:10.3748/wjg.15.1259

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 15, Issue 10

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (1626)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-3) series, Tables (1-1) series.

Item

Count

PDF

268

HTML

1122

Figures (1-3)

115

Tables (1-1)

121

Sum=1626

Mar 14, 2009 (publication date) through Apr 23, 2024

Times Cited of This Article

Times Cited (3)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Article

World J Gastroenterol. Mar 14, 2009; 15(10): 1259-1263
Published online Mar 14, 2009. doi: 10.3748/wjg.15.1259

Antisense expression of PKCα improved sensitivity of SGC7901/VCR cells to doxorubicin

Da-Long Wu, Feng-Ying Sui, Cheng Du, Cheng-Wen Zhang, Bin Hui, Shui-Ling Xu, Huan-Zhang Lu, Guo-Jie Song

Da-Long Wu, Feng-Ying Sui, Cheng-Wen Zhang, Bin Hui, Guo-Jie Song, Department of Pharmacology, School of Medicine, Jiaxing College, Jiaxing 314001, Zhejiang Province, China

Cheng Du, Traditional Chinese Medicine Hospital of Jiaxing City, Jiaxing 314001, Zhejiang Province, China

Shui-Ling Xu, Department of Microbiology, School of Medicine, Jiaxing College, Jiaxing 314001, Zhejiang Province, China

Huan-Zhang Lu, Department of Clinical Pharmacology, Affiliated Hospital, Chinese Academy of Military Medical Sciences, Beijing 100039, China

Author contributions: Wu DL, Zhang CW and Hui B performed the research; Sui FY, Du C, Xu SL and Song GJ gave some advice; Wu DL and Lu HZ designed the research; Wu DL wrote the paper.

Correspondence to: Da-Long Wu, PhD, Associate Professor, Department of Pharmacology, School of Medicine, Jiaxing College, Jiaxing 314001, Zhejiang Province, China. wudalong66@sina.com

Telephone: +86-573-83643836

Fax: +86-573-83643497

Received: August 1, 2008
Revised: October 25, 2008
Accepted: November 1, 2008
Published online: March 14, 2009

Abstract

AIM: To explore whether antisense blocking of protein kinase C alpha (PKCα) would reverse multi-drug resistance (MDR) in the vincristine (VCR)-resistant human gastric cancer cell line SGC7901/VCR.

METHODS: SGC7901/VCR cells expressing antisense PKCα, SGC7901/VCR/aPKC, were established by transfection with a recombinant plasmid reversely inserted with PKCα cDNA. Empty vector (PCI-neo)-transfected cell clones, SGC7901/VCR/neo, served as the control. Western blot method was used to detect PKCα content in SGC7901, SGC7901/VCR, SGC7901/VCR/neo and SGC7901/VCR/aPKC cells, using PKCα-specific antibody. The sensitivity of SGC7901, SGC7901/VCR, SGC7901/VCR/neo and SGC7901/VCR/aPKC cells to doxorubicin (DOX) in vitro was determined by MTT assay. The uptake of DOX in these cells was detected with fluorescence spectrophotometer.

RESULTS: Western blot analysis showed that the PKCα protein level was about 8.7-fold higher in SGC7901/VCR cells than that in SGC7901 cells, whereas the protein expression of PKCα was reduced by 78% in SGC7901/VCR/aPKC cells when compared with the SGC7901/VCR cells. SGC7901/VCR/aPKC cells had a 4.2-fold increase in DOX cytotoxicity, accompanied by a 1.7-fold increase of DOX accumulation in comparison with SGC7901/VCR cells.

CONCLUSION: PKCα positively regulates MDR in SGC7901 cells, and inhibition of PKCα can partially attenuate MDR in human gastric cancer cells.

Keywords: Multi-drug resistance, Protein kinase C alpha, SGC7901, Gastric cancer