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Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Dec 14, 2008; 14(46): 7107-7111
Published online Dec 14, 2008. doi: 10.3748/wjg.14.7107
Identification of human papillomavirus in esophageal squamous papillomas
Olga L Bohn, Leticia Navarro, Jesus Saldivar, Sergio Sanchez-Sosa
Olga L Bohn, Leticia Navarro, Jesus Saldivar, Sergio Sanchez-Sosa, Department of Pathology, UPAEP University Hospital, 5 Poniente 715, CP72000, Puebla, Mexico
Author contributions: Bohn OL and Sanchez-Sosa S wrote the paper, performed the research, collected data and analyzed the data; Navarro L and Saldivar J performed the research.
Correspondence to: Dr. Olga L Bohn, MD, Department of Pathology, Case Western Reserve University-MetroHealth Medical Center, 2500 MetroHealth Dr, Cleveland OH 44109, United States. omejiam@gmail.com
Telephone: +1-412-805-2097 Fax: +1-216-778-5701
Received: September 14, 2008
Revised: November 19, 2008
Accepted: November 26, 2008
Published online: December 14, 2008

AIM: To investigate the presence of human papillomavirus (HPV) in esophageal squamous papilloma (ESP) and determine p16, p53 and Ki67 expression in a Mexican cohort.

METHODS: Nineteen cases diagnosed as ESP, corresponding to 18 patients were reviewed; nineteen cases of normal esophageal mucosa were used as negative controls. HPV detection was performed by amplified chromogenic in situ hybridization (ACISH) using a wide spectrum-cocktail probe and PCR.

RESULTS: The average age at presentation was 46.3 years (range 28-72 years). Patients included four (22.22%) males and 14 (77.77%) females. The most frequent location was upper third (11 cases), followed by middle third (3 cases) and unknown site (5 cases). Immunohistochemistry (IHC) revealed basal and focal p53 expression in 17 cases (89%); p16 was expressed in eight cases (42.10%) and the Ki67 index ranged from 10% to 30%. HPV was detected in 14 out of 16 cases (87.5%) by ACISH: Twelve showed diffuse nuclear patterns and two showed granular patterns. HPV DNA was identified by PCR in 12 out of 14 cases (85.7%). Low-risk HPV types were detected in the most of the cases.

CONCLUSION: This study provides identification of HPV infection in almost 80% of ESP using either ACISH or PCR; overall, all of these lesions show low expression of cell-cycle markers. We suggest ACISH as an alternative diagnostic tool for HPV detection in ESP.

Keywords: Human papillomavirus, Esophageal papilloma, Papillomatosis, Esophageal neoplasm, Immunohistochemistry, Cell cycle