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Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Aug 7, 2008; 14(29): 4677-4683
Published online Aug 7, 2008. doi: 10.3748/wjg.14.4677
Blood F2-isoprostanes are significantly associated with abnormalities of lipid status in rats with steatosis
Man-Jie Zhu, Li-Juan Sun, Yue-Qin Liu, Yan-Ling Feng, Hai-Tao Tong, Ying-He Hu, Zheng Zhao
Man-Jie Zhu, Li-Juan Sun, Yue-Qin Liu, Ying-He Hu, Zheng Zhao, Laboratory of Pharmacology, Institute of Brain Functional Genomics, East China Normal University, Key Laboratory of Brain Functional Genomics, MOE & STCSM, Shanghai 200062, China
Yan-Ling Feng, Hai-Tao Tong, Department of Pathology, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Author contributions: Zhu MJ and Zhao Z designed research; Zhu MJ, Sun LJ, Liu YQ, Feng YL and Tong HT performed research; Zhu MJ, Hu YH and Zhao Z analyzed the data and wrote the paper.
Correspondence to: Dr. Zheng Zhao, Shanghai Institute of Brain Functional Genomics, East China Normal University, 3663 Zhongshan North Road, Shanghai 200062, China. zzhao@brain.ecnu.edu.cn
Telephone: +86-21-62238005
Fax: +86-21-62601953
Received: February 4, 2008
Revised: June 27, 2008
Accepted: July 4, 2008
Published online: August 7, 2008
Abstract

AIM: To investigate oxidative stress and lipid peroxidation in hepatic steatosis and the underlying implications in pathological mechanisms of non-alcoholic fatty liver disease (NAFLD).

METHODS: F2-isoprostanes (iPF-III) in blood and liver samples from steatotic (n = 9) and control (n = 7) rats were measured as in vivo marker of lipid peroxidation by a mass spectrometric approach. The lipid profile and endogenous antioxidant status (SOD and CAT) in the rats were also analyzed.

RESULTS: Significantly higher levels of iPF-III (mean 3.47 vs 2.40 pmol/mg tissue, P = 0.004) and lower activities of SOD (mean 1.26 U vs 1.40 U, P < 0.001) and CAT (mean 1026.36 U/mg vs 1149.68 U/mg protein, without significance) were observed in the livers of steatotic rats. Plasma total iPF-III was significantly correlated with the abnormalities of blood lipids as well as alanine aminotransferase (ALT) levels in the rats with simple steatosis, whereas no similar tendencies were observed in the control rats.

CONCLUSION: Enhancement of hepatic oxidative imbalance occurring at the steatotic stage of NAFLD suggests a possibility that manifestation of the local oxidative damage precedes that of systemic oxidative imbalance. Predominant metabolic features of the increased lipid peroxidation further suggest a close association of the oxidative imbalance and the dyslipidemia with functional deterioration of the steatotic liver. The findings need to be further evaluated, especially in human studies.

Keywords: Isoprostanes, Oxidative stress, Lipid peroxidation, Steatosis, Non-alcoholic fatty liver disease