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Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Jan 14, 2008; 14(2): 243-247
Published online Jan 14, 2008. doi: 10.3748/wjg.14.243
Promoter polymorphism of transforming growth factor-β1 gene and ulcerative colitis
B Tamizifar, KB Lankarani, S Naeimi, M Rismankar Zadeh, A Taghavi, A Ghaderi
B Tamizifar, KB Lankarani, M Rismankar Zadeh, A Taghavi, Department of Internal Medicine, Namazee Hospital, Shiraz, Iran
S Naeimi, A Ghaderi, Shiraz Institute of Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Correspondence to: Kamran Bagheri Lankarani, Department of Internal Medicine, Nemazi hospital, Zand Ave, Shiraz, Iran. lankaran@sums.ac.ir
Telephone: +98-713-2301452
Fax: +98-711-2304952
Received: February 20, 2006
Revised: November 3, 2007
Published online: January 14, 2008

AIM: To elucidate the possible difference in two promoter polymorphisms of the transforming growth factor-β1 (TGF-β1) gene (-800G > A, -509C > T) between ulcerative colitis (UC) patients and normal subjects.

METHODS: A total of 155 patients with established ulcerative colitis and 139 normal subjects were selected as controls. Two single nucleotide polymorphisms within the promoter region of TGF-β1 gene (-509C > T and -800G > A) were genotyped using PCR-RFLP.

RESULTS: There was a statistically significant difference in genotype and allele frequency distributions between UC patients and controls for the -800G > A polymorphism of the TGF-β1 gene (P < 0.05). The frequency of the TGF-β1 gene polymorphism at position -800 showed that the AA genotype and the allele A frequencies significantly differed between the patients and healthy controls (P < 0.05). At position -509, there was no statically significant difference in genotype and allele frequency between the patients and control subjects.

CONCLUSION: The results of our study indicate that there is a significant difference in both allele and genotype frequency at position -800G > A of TGF-β1 gene promoter between Iranian patients with UC and normal subjects.

Keywords: Transforming growth factor-β1, Ulcerative colitis, Promoter, Polymorphism, Iran