Gong J, Lao XJ, Wang XM, Long G, Jiang T, Chen S. Preservation of non-heart-beating donor livers in extracorporeal liver perfusion and histidine-trytophan-ketoglutarate solution. World J Gastroenterol 2008; 14(15): 2338-2342 [PMID: 18416459 DOI: 10.3748/wjg.14.2338]
Corresponding Author of This Article
Jin Gong, Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China. gongjin622@yahoo.com.cn
Article-Type of This Article
Basic Research
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World J Gastroenterol. Apr 21, 2008; 14(15): 2338-2342 Published online Apr 21, 2008. doi: 10.3748/wjg.14.2338
Preservation of non-heart-beating donor livers in extracorporeal liver perfusion and histidine-trytophan-ketoglutarate solution
Jin Gong, Xue-Jun Lao, Xi-Mo Wang, Gang Long, Tao Jiang, Shi Chen
Jin Gong, Xue-Jun Lao, Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China
Xi-Mo Wang, Gang Long, Tao Jiang, Department of Surgery, Tianjin People’s Hospital, Tianjin 300120, China
Shi Chen, Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Author contributions: Gong J and Wang XM contributed equally to this work; Gong J, Lao XJ, Wang XM, Long G, Jiang T and Chen S designed the research; Gong J, Lao XJ, Wang XM and Long G performed the research; Wang XM contributed to new reagents/analytic tools; Gong J and Lao XJ analyzed the data; Gong J wrote the paper.
Correspondence to: Jin Gong, Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China. gongjin622@yahoo.com.cn
Telephone: +86-20-38688613
Fax: +86-20-85223255
Received: November 28, 2007 Revised: February 22, 2008 Published online: April 21, 2008
Abstract
AIM: To compare the preservation of non-heart-beating donor (NHBD) livers in cold histidine-trytophan-ketoglutarate (HTK) solution and extracorporeal liver perfusion (ECLP).
METHODS: Livers harvested from health pigs were stored for 10 h in cold HTK solution (group A, n = 4) or perfused with oxygenated autologous blood at body temperature (group B, n = 4). Both groups were then tested on the circuit for 4 h. Bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of extracorporeal livers were tested in each group. Liver tissues from each group were examined at the end of reperfusion.
RESULTS: At 1, 2, 3 and 4 h after reperfusion, bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of livers in group A were statistically different from those in group B (P < 0.05 or P < 0.01).
CONCLUSION: ECLP is better than HTK solution to preserve NHBD livers. ECLP can assess the graft viability before liver transplantation.
