Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1300
Revised: July 10, 2005
Accepted: July 12, 2005
Published online: February 28, 2006
AIM: To establish a new, reliable vomit model of minks.
METHODS: Adult male minks were randomly divided into 8 groups (n = 6): cisplatin (7.5 mg/kg) intraperitoneal injection (ip) group, copper sulfate (40 mg/kg) intragastric injection (ig) group, apomorphine (1.6 mg/kg) subcutaneous injection (sc) group, and 18 Gy whole-body X-irradiation group, ondansetron injection group (2 mg/kg ip) 30 min later followed by cisplatin (7.5 mg/kg) ip, normal saline (NS) ip injection control group, metoclopramide injection group (4 mg/kg ip) 30 min later followed by apomorphine (1.6 mg/kg) sc, NS ig control group. The frequency of retching and vomiting was calculated. After behavioral experiment, distribution of 5-HT in the ileum was detected by immunohistologic method.
RESULTS: Cisplatin, apomorphine, copper sulfate and X-irradiation administered to minks evoked a profound emetic response in the animals. However, retching and vomiting were significantly inhibited by pretreatment with ondansetron and metoclopramide in cisplatin and copper sulfate groups (P = 0.018). Immunohistologic result showed that 5-HT released from enterochromaffin cells (EC cells) was involved in vomiting mechanism.
CONCLUSION: Mink vomit model has a great value in studying the vomiting mechanism and screening new antiemetic drugs.