Flow cytometry assay of myeloid dendritic cells (mDCs) in peripheral blood during acute hepatitis C: Possible pathogenetic mechanisms

doi:10.3748/wjg.v12.i7.1105

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Feb 21, 2006 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 21, 2006; 12(7): 1105-1109
Published online Feb 21, 2006. doi: 10.3748/wjg.v12.i7.1105

Flow cytometry assay of myeloid dendritic cells (mDCs) in peripheral blood during acute hepatitis C: Possible pathogenetic mechanisms

Alessandro Perrella, Luigi Atripaldi, Pasquale Bellopede, Tommaso Patarino, Costanza Sbreglia, Giovanni Tarantino, Paolo Sorrentino, Paolo Conca, Luca Ruggiero, Oreste Perrella

Alessandro Perrella, Luigi Atripaldi, Pasquale Bellopede, Tommaso Patarino, Costanza Sbreglia, Oreste Perrella, Department Infectious Disease and Immunology, Hospital D.Cotugno, Naples, Italy

Giovanni Tarantino, Paolo Sorrentino, Paolo Conca, Department Clinical and Experimental Medicine, Federico II Medical School University

Luca Ruggiero, Institute for Cancer Genetics, Columbia University

Co-first-author: Luigi Atripaldi

Correspondence to: Alessandro Perrella, MD, Department Infectious Disease and Immunology, Hospital D.Cotugno, Via E.A. Mario 35 , Zip code 80131, Naples, Italy. alex.perrel@virgilio.it

Telephone: +39-347-3356089 Fax: +39-81-5608257

Received: August 1, 2005
Revised: October 6, 2005
Accepted: October 26, 2005
Published online: February 21, 2006

Abstract

AIM: To asses the expression of myeloid dendritic cells (CD11c+) subset during acute HCV hepatitis and its possible involvement in natural history of the infection.

METHODS: We enrolled 11 patients with acute hepatitis C (AHC) (Group A), 10 patients with acute hepatitis A (AHA) (as infective control-Group B) and 10 healthy donors (group C) in this study. All patients underwent selective flow cytometry gating strategies to assess the peripheral number of the myeloid dendritic cells (mDCs) to understand the possible role and differences during acute hepatitis.

RESULTS: Eight of 11 patients with acute HCV hepatitis did not show any increase of mDCs compared to healthy individuals, while a significant decrease of mDCs was found in absolute cell count (z = -2.37; P < 0.05) and percentage (z= -2.30; P < 0.05) as compared with AHA. On the contrary, The remaining three patients of the group A had a higher mDCs number and percentage as occur in group B. Interestingly, after six months, those patients did not show any increase of mDCs subset were chronically infected. while the three subjects with an increase of peripheral mDCs, as in HAV acute infection, resolved the illness.

CONCLUSION: The lack of increase of mDCs during acute hepatitis C might be an important factor involved in chronicization of the infection.

Keywords: Myeloid dendritic cells, HCV, CD4+, CD11c+, HCV-RNA, HAV