Lim SB, Jeong SY, Kim IJ, Kim DY, Jung KH, Chang HJ, Choi HS, Sohn DK, Kang HC, Shin Y, Jang SG, Park JH, Park JG. Analysis of microsatellite instability in stool DNA of patients with colorectal cancer using denaturing high performance liquid chromatography. World J Gastroenterol 2006; 12(41): 6689-6692 [PMID: 17075985 DOI: 10.3748/wjg.v12.i41.6689]
Corresponding Author of This Article
Jae-Gahb Park, MD, PhD, Cancer Research Institute and Cancer Research Center, Seoul National University, Seoul, Korea. jgpark@plaza.snu.ac.kr
Article-Type of This Article
Rapid Communication
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World J Gastroenterol. Nov 7, 2006; 12(41): 6689-6692 Published online Nov 7, 2006. doi: 10.3748/wjg.v12.i41.6689
Analysis of microsatellite instability in stool DNA of patients with colorectal cancer using denaturing high performance liquid chromatography
Seok-Byung Lim, Seung-Yong Jeong, Il-Jin Kim, Dae Yong Kim, Kyung Hae Jung, Hee Jin Chang, Hyo Seong Choi, Dae Kyung Sohn, Hio Chung Kang, Yong Shin, Sang-Geun Jang, Jae-Hyun Park, Jae-Gahb Park
Seok-Byung Lim, Seung-Yong Jeong, Dae Yong Kim, Kyung Hae Jung, Hee Jin Chang, Hyo Seong Choi, Dae Kyung Sohn, Jae-Gahb Park, Research Institute and Hospital, National Cancer Center, Goyang, Korea
Il-Jin Kim, Hio Chung Kang, Yong Shin, Sang-Geun Jang, Jae-Hyun Park, Jae-Gahb Park, Cancer Research Institute and Cancer Research Center, Seoul National University, Seoul, Korea
Jae-Gahb Park, Department of Surgery, Seoul National University College of Medicine, Seoul, Korea
Supported by research grant from the National Cancer Center, Korea, No.0410063-3
Correspondence to: Jae-Gahb Park, MD, PhD, Cancer Research Institute and Cancer Research Center, Seoul National University, Seoul, Korea. jgpark@plaza.snu.ac.kr
Telephone: +82-2-20723380 Fax: +82-2-7424727
Received: April 27, 2006 Revised: August 12, 2006 Accepted: September 14, 2006 Published online: November 7, 2006
Abstract
AIM: To evaluate the usefulness of denaturing high performance liquid chromatography (DHPLC) for analyzing microsatellite instability (MSI) status in stool DNA of patients with colorectal cancer.
METHODS: A total of 80 cancer tissues from patients with primary sporadic colorectal tumor (proximal cancer: 27, distal cancer: 53) and matched stool (which were employed for comparison with the tissues) were analyzed for MSI status in BAT 26. DNA samples extracted from stool were evaluated by nested polymerase chain reaction (PCR) and DHPLC for MSI analysis.
RESULTS: Six cases (7.5%) of MSI were identified in BAT 26 from 80 cancer tissues. All the stool DNA samples from patients whose cancer tissue showed MSI also displayed MSI in BAT 26.
CONCLUSION: As MSI is one of the established fecal DNA markers to screen colorectal cancer, we propose to use DHPLC for the MSI analysis in fecal DNA.
