Gastric Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 28, 2006; 12(32): 5135-5139
Published online Aug 28, 2006. doi: 10.3748/wjg.v12.i32.5135
Increased expression of angiogenin in gastric carcinoma in correlation with tumor angiogenesis and proliferation
Yu Chen, Sheng Zhang, Yu-Peng Chen, Jian-Yin Lin
Yu Chen, Department of Physiology and Pathophysiology, Fujian Medical University, Fuzhou 350004, Fujian Province, China
Sheng Zhang, Yu-Peng Chen, Department of Pathology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China
Jian-Yin Lin, Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350004, Fujian Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Science Foundation of Fujian Province, No.C0110013, and Science and Technology KeyProgram Foundation of Fujian Province, No. 2002Y003
Correspondence to: Professor Jian-Yin Lin, Department of Biochemistry and Molecular Biology, Fujian Medical University, 88 Jiaotong Road, Fuzhou 350004, Fujian Province, China. jylin@mail.fjmu.edu.cn
Telephone: +86-591-83348087 Fax: +86-591-83569132
Received: April 13, 2006
Revised: May 18, 2006
Accepted: May 25, 2006
Published online: August 28, 2006
Abstract

AIM: To investigate the implication of angiogenin (ANG) in the neovascularizaton and growth of human gastric carcinoma (HGC).

METHODS: ANG mRNA expression in HGC specimens obtained by surgical resection from patients with HGC were examined by RT-PCR. ANG, Ki-67, VEGF protein expression and microvessel density (MVD) in HGC specimens were detected by immunohistochemistry.

RESULTS: RT-PCR showed significantly higher ANG mRNA expression (0.482 ± 0.094) in HGC tissues than in the surrounding nontumorous tissues (0.276 ± 0.019, P = 0.03). MVD within tumorous tissues increased significantly with ANG mRNA expression (r = 0.380, P = 0.001) and ANG protein expression (P < 0.01). The ANG expression levels of cancer tissues were positively correlated with VEGF (P < 0.01) and the proliferation index of cancer cells (P < 0.01).

CONCLUSION: ANG is one of the neovascularization factors of HGC. ANG may work in coordination with VEGF, and promote the proliferation of HGC cells.

Keywords: Angiogenin, Gastric carcinoma, Vascular endothelial growth factor, Angiogenesis