Differentiation of hepatocytoid cell induced from whole-bone-marrow method isolated rat myeloid mesenchymal stem cells

doi:10.3748/wjg.v12.i30.4866

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Aug 14, 2006 (publication date) through Sep 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 14, 2006; 12(30): 4866-4869
Published online Aug 14, 2006. doi: 10.3748/wjg.v12.i30.4866

Differentiation of hepatocytoid cell induced from whole-bone-marrow method isolated rat myeloid mesenchymal stem cells

Wei Li, Su-Nan Liu, Duan-De Luo, Lei Zhao, Ling-Lan Zeng, Shu-Ling Zhang, Shu-Li Li

Wei Li, Su-Nan Liu, Duan-De Luo, Lei Zhao, Ling-Lan Zeng, Shu-Ling Zhang, Shu-Li Li, Department of Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Wei Li, Department of Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. lsnlw@sina.com

Telephone: +86-27-62760325

Received: April 13, 2006
Revised: May 1, 2006
Accepted: May 25, 2006
Published online: August 14, 2006

Abstract

AIM: To explore the expansion and differentiation of hepatocytoid cell induced from myeloid mesenchymal stem cell (MSC) in vitro, in order to find suitable resource of hepatocytes for bioartificial liver or liver transplantation.

METHODS: The rat myeloid MSC was isolated and divided into three groups which were cultured by Friedensteion method, and then were induced by culture fluid, culture fluid plus cholestatic serum and culture fluid plus hepatocyte growth factor (HGF), respectively. Hepatocytoid cell as well as expression of CK18 and AFP was observed by immunohistochemistry.

RESULTS: After the induction for 21 d, hepatocytoid cell was observed, and its expression of CK18 and AFP was detected by immunohistochemistry in MSC cultured with cholestatic serum. Furthermore, on the 35^th d, albumin mRNA was expressed in the cell, suggesting the inducing effect was similar to that by HGF.

CONCLUSION: Rat myeloid MSC can differentiate into hepatocyte lineage under appropriate condition. This method is easy to operate.

Keywords: Mesenchyme stem cell; Hepatocytoid cell; Expansion; Differentiation; Induction