Wang YF, Guo KJ, Huang BT, Liu Y, Tang XY, Zhang JJ, Xia Q. Inhibitory effects of antisense phosphorothioate oligodeoxynucleotides on pancreatic cancer cell Bxpc-3 telomerase activity and cell growth in vitro. World J Gastroenterol 2006; 12(25): 4004-4008 [PMID: 16810748 DOI: 10.3748/wjg.v12.i25.4004]
Corresponding Author of This Article
Dr. Yun-Feng Wang, Department of General Surgery, The Center Hospital of Shanghai Yangpu District (Branch Hospital of the Affiliated Xinhua Hospital of Shanghai Jiaotong University), Shanghai 200127, China. wangyunfeng197911@hotmail.com
Article-Type of This Article
Basic Research
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Yun-Feng Wang, Department of General Surgery, The Center Hospital of Shanghai Yangpu District (Branch Hospital of The Affiliated Xinhua Hospital of Shanghai Jiaotong University), Shanghai 200127, China
Jian-Jun Zhang, Qiang Xia, Center of Liver Transplantation of The Affiliated Renji Hospital of Shanghai Jiaotong University, Shanghai 200127, China
Ke-Jian Guo, Yong Liu, Department of Biliary-Pancreatic Surgery, The First Affiliated Hospital of China Medical University, Shengyang 110001, Liaoning Province, China
Bei-Ting Huang, Department of Clinical Epidemiology, Staff Room of the First Affiliated Hospital of China Medical University, Shengyang 11001, Liaoning Province, China
Xiao-Yun Tang, The First Department of The Center of Experiment and Technology, The China Medical University, Shengyang 11001, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Yun-Feng Wang, Department of General Surgery, The Center Hospital of Shanghai Yangpu District (Branch Hospital of the Affiliated Xinhua Hospital of Shanghai Jiaotong University), Shanghai 200127, China. wangyunfeng197911@hotmail.com
Telephone: +86-13370291239 Fax: +86-21-68732693
Received: May 17, 2005 Revised: July 5, 2005 Accepted: July 15, 2005 Published online: July 7, 2006
Abstract
AIM: To investigate the effect of telomerase hTERT gene antisense oligonucleotide (hTERT-ASO) on proliferation and telomerase activity of pancreatic cancer cell line Bxpc-3.
METHODS: MTT assay was used to detect the effect of different doses of hTERT-ASO on proliferation of Bxpc-3 cell for different times. To study the anti-tumor activity, the cells were divided into there groups: Control group (pancreatic cancer cell Bxpc-3); antisense oligonucleotide (hTERT-ASO) group; and nosense oligonucleotide group decorated with phosphorothioate. Telomerase activity was detected using TRAP-PCR-ELISA. Cell DNA distribution was examined using flow cytometry assay. Cell apoptosis was observed by transmission electron microscope in each group.
RESULTS: After treatment with 6 mmol/L hTERT-ASO, cell proliferation was inhibited in dose- and time-dependent manner. The telomerase activity decreased after treatment with hTERT-ASO for 72 h. Flow cytometry showed the cell number of G0/G1 phase increased from 2.7% to 14.7%, the cell number of S phase decreased from 72.7% to 51.0%, and a sub-G1 stage cell apoptosis peak appeared in front of G1 stage.
CONCLUSION: Telomerase antisense oligodeoxy-nucleotide can inhibit the proliferation of pancreatic cancer cell line Bxpc-3 and decrease the telomerase activity and increase cell apoptosis rate in vitro.
