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World J Gastroenterol. Feb 14, 2005; 11(6): 885-889
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.885
Gene expression profile in rat small intestinal allografts after cold preservation/reperfusion
Shu-Feng Wang, Qi Liang, Guo-Wei Li, Kun Gao
Shu-Feng Wang, Department of General Surgery, First Hospital, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Qi Liang, Department of Anesthesiology, Xi’an traditional Chinese Hospital, Xi’an 710001, Shaanxi Province, China
Guo-Wei Li, Department of General Surgery, Second Hospital, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Kun Gao, School of Life Science, Xi’an Jiaotong University, Xi’an 710049, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30271275
Correspondence to: Dr. Shu-Feng Wang, Department of General Surgery, First Hospital, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. dawnwsf@sina.com
Telephone: +86-29-85324147 Fax: +86-29-82655730
Received: April 14, 2004
Revised: April 17, 2004
Accepted: May 13, 2004
Published online: February 14, 2005
Abstract

AIM: To determine the changes of gene expression profile in small intestinal allografts in rats after cold preservation/reperfusion, and to identify the genes relevant to cold preservation/reperfusion injury.

METHODS: Heterotopic segmental small bowel transpla-ntation was performed in six rats with a sham operation and they were used as controls. Total RNA was extracted from the allografts (experimental group) and normal intestines (control group) 1 h after cold preservation/reperfusion, and then purified to mRNA, which was then reversely transcribed to cDNA, and labeled with fluorescent Cy5-dUTP and Cy3-dUTP to prepare hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high-stringent washing, the fluorescent signals on cDNA microarray chip were scanned and analyzed.

RESULTS: Among the 4 096 target genes, 82 differentially expressed genes were identified between the two groups. There were 18 novel genes, 33 expression sequence tags, and 31 previously reported genes. The selected genes may be divided into four classes: genes modulating cellular adhesion, genes regulating cellular energy, glucose and protein metabolism, early response genes and other genes.

CONCLUSION: A total of 82 genes that may be relevant to cold preservation/reperfusion injury in small intestinal allografts are identified. Abnormal adhesion between polymorphonuclears and endothelia and failure in energy, glucose and protein metabolism of the grafts may contribute to preservation/reperfusion injury. The functions of the novel genes identified in our study need to be clarified further.

Keywords: Small intestinal allografts; Cold preservation; Reperfusion Injury; Gene Expression Profiling