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World J Gastroenterol. Dec 7, 2005; 11(45): 7203-7207
Published online Dec 7, 2005. doi: 10.3748/wjg.v11.i45.7203
Synergistic action of famotidine and chlorpheniramine on acetic acid-induced chronic gastric ulcer in rats
Zhen Qin, Chao Chen
Zhen Qin, Chao Chen, Laboratory of natural drugs of Medical College, China Three Gorges University, Yichang 443002, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Supported by Scientific and Technological Offices of Yichang Municipality, No A03210
Correspondence to: Professor Chao Chen, Laboratory of Natural Drugs of Medical College, China Three Gorges University, Yichang 443002, Hubei Province, China. chaochen1954@163.com
Telephone: +0717-6397466
Received: April 15, 2005
Revised: May 23, 2005
Accepted: May 25, 2005
Published online: December 7, 2005
Abstract

AIM: To assess the synergistic action of famotidine (FMD) and chlorpheniramine (CPA) on acetic acid-induced chronic gastric ulcer in rats.

METHODS: Chronic gastric lesions were induced in male Sprague-Dawley (SD) rats by serosal application of the acetic acid. Forty SD rats were randomly divided into blank group (n = 8), control group (n = 8), FMD group (n = 8), CPA group (n = 8), and FMD+CPA group (n = 8). Each group was given intraperitoneally (i.p.) 0.5 mL/100 g distilled water, 9 g/L NaCl saline, 4 mg/kg FMD, 10 mg/kg CPA, 4 mg/kg FMD+10 mg/kg CPA, respectively, daily for 10 d. On d 10, ulcer area was determined by planimetry. The level of myeloperoxidase (MPO) in the liver homogenation was determined by biochemical methods and the plasma levels of 6-ketoprostaglandin F1 alpha (6-keto-PGF1a) and IL-8 were determined by radioimmunoassay.

RESULTS: The synergistic effects of FMD+CPA group on the lesion, IL-8, 6-keto-PGF1a and MPO were confirmed. The effect of FMD+CPA group was significantly different as compared to the control and FMD groups. The lesion (mm2) was reduced from 40.18±2.6 in control group to 6.83±2.97 in PMD+CPA group, P<0.01, and from 32.9±3.27 in FMD group to 6.83±2.97 in PMD+CPA group, P<0.01. The plasma levels of IL-8 decreased from 0.69±0.11 ng/L in control group to 0.4±0.04 ng/L in PMD+CPA group, P<0.01, and from 0.51±0.08 ng/L in FMD group to 0.4±0.04 ng/L in PMD+CPA group, P<0.05. The level of 6-keto-PGF1a increased from 7.55±1.65 ng/L in control group to 16.62±0.97 ng/L in PMD+CPA group, P<0.01, and from 13.15±1.48 ng/L in FMD group to 16.62±0.97 ng/L in PMD+CPA group, P<0.05. The levels of MPO in the liver homogenate decreased from 9.12±2.05 u/L in control group to 4.33±0.95 u/L in PMD+CPA group, P<0.01, and from 8.3±1.29 u/L in FMD group to 4.33±0.95 u/L, P<0.01.

CONCLUSION: The synergistic action of FMD and CPA on acetic acid-induced chronic gastric ulcer in rats decreases the incidence of ulcer and also enhances the healing of ulcer.

Keywords: Gastric ulcer, Acetic acid, Famotidine, Chlorpheniramine, Interleukin-8, 6-Ketoprostaglandin F1 alpha, Myeloperoxidase