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World J Gastroenterol. Nov 28, 2005; 11(44): 7040-7043
Published online Nov 28, 2005. doi: 10.3748/wjg.v11.i44.7040
Cell survival curve for primary hepatic carcinoma cells and relationship between SF2 of hepatic carcinoma cells and radiosensitivity
Zhi-Zhong Liu, Wen-Ying Huang, Ju-Sheng Lin, Xiao-Sheng Li, Xiao Lan, Xiao-Kun Cai, Kuo-Huan Liang, Hai-Jun Zhou
Zhi-Zhong Liu, Ju-Sheng Lin, Xiao-Kun Cai, Kuo-Huan Liang, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Wen-Ying Huang, Hai-Jun Zhou, The People’s Hospital of Chenzhou, Chenzhou 423000, Hunan Province, China
Xiao-Sheng Li, Xiao Lan, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr Zhi-Zhong Liu, Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. whzzl@163.net
Telephone: +86-27-13554460933
Received: June 18, 2004
Revised: August 8, 2004
Accepted: August 12, 2004
Published online: November 28, 2005
Abstract

AIM: To establish the cell survival curve for primary hepatic carcinoma cells and to study the relationship between SF2 of primary hepatic carcinoma cells and radiosensitivity.

METHODS: Hepatic carcinoma cells were cultured in vitro using 39 samples of hepatic carcinoma at stages II-IV. Twenty-nine samples were cultured successfully in the fifth generation cells. After these cells were radiated with different dosages, the cell survival ratio and SF2 were calculated by clonogenic assay and SF2 model respectively. The relationship between SF2 and the clinical pathological feature was analyzed.

RESULTS: Twenty-nine of thirty-nine samples were successfully cultured. After X-ray radiation of the fifth generation cells with 0, 2, 4, 6, 8 Gy, the cell survival rate was 41%, 36.5%, 31.0%, 26.8%, and 19%, respectively. There was a negative correlation between cell survival and irradiation dosage (r = -0.973, P<0.05). SF2 ranged 0.28-0.78 and correlated with the clinical stage and pathological grade of hepatic carcinoma (P<0.05). There was a positive correlation between SF2 and D0.5 (r = 0.773, P<0.05).

CONCLUSION: SF2 correlates with the clinical stage and pathological grade of hepatic carcinoma and is a marker for predicting the radiosensitivity of hepatic carcinomas.

Keywords: Hepatocarcinoma; SF2; Radiosensitivity; D0.54