Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 28, 2005; 11(4): 587-592
Published online Jan 28, 2005. doi: 10.3748/wjg.v11.i4.587
Effect of vitamin E and human placenta cysteine peptidase inhibitor on expression of cathepsins B and L in implanted hepatoma Morris 5123 tumor model in Wistar rats
Tadeusz Sebzda, Piotr Hanczyc, Yousif Saleh, Bernice F Akinpelumi, Maciej Siewinski, Jerzy Rudnicki
Tadeusz Sebzda, Piotr Hanczyc, Department of Pathophysiology, Medical University of Wroclaw, Poland
Yousif Saleh, Bernice F Akinpelumi, Department of Obstetrics and Gynaecology, Medical University of Wroclaw, Poland
Maciej Siewinski, Faculty of Public Health, Medical University of Wroclaw, Poland
Jerzy Rudnicki, Clinical of Surgery and Oncology, Medical University of Wroclaw, Poland
Author contributions: All authors contributed equally to the work.
Correspondence to: Yousif Saleh, PhD, I Department of Obstetrics and Gynaecology, Medical University of Wroclaw, Chalubinskiego street 3; PL-50-368 Wroclaw, Poland.
Telephone: +4871-7842413
Received: February 28, 2004
Revised: March 3, 2004
Accepted: May 13, 2004
Published online: January 28, 2005

AIM: To examine the effectiveness of human placental inhibitors, by injecting vitamin E to rats with transplanted Morris-5123 hepatoma, on the expression of cathepsins B and L in tumor, liver, lung and blood sera after transplantation of Morris 5123 hepatoma.

METHODS: Animals were divided into 10 groups receiving three different concentrations of vitamin E and inhibitors along or in combination and compared with negative control (healthy rats) and positive control (tumor rats). Effectiveness of treatment was evaluated with regard to survival time, tumor response and determination of the activities of proteolytic enzymes and their inhibitors using flurogenic substrates.

RESULTS: Cathepsins B and L activities were elevated by 16-fold in comparison with negative control tissues, and their endogenous inhibitor activity decreased by 1.2-fold before treatment. In several cases, tumors completely disappeared following vitamin E plus human placental cyteine protease inhibitor (CPI) compared with controls. The number of complete tumor responses was higher when 20 m/kg vitamin E plus 400 μg of CPI was used, i.e., 7/10 rats survived more than two mo. Cathepsins B and L were expressed significantly in tumor, liver, lung tissues and sera in parallel to the increasing of the endogenous inhibitor activity compared with the controls after treatment (P<0.0001).

CONCLUSION: The data indicate formation of metastasis significantly reduced in treated rats, which might provide a therapeutic basis for anti-cancer therapy.

Keywords: Morris-5123 hepatoma, Vitamin E, Human placenta cysteine peptidase inhibitor, Cathepsin B, Cathepsin L