Basic Research
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 7, 2005; 11(37): 5782-5786
Published online Oct 7, 2005. doi: 10.3748/wjg.v11.i37.5782
Effect of Astragalus complanatus flavonoid on anti-liver fibrosis in rats
Chun-Yu Liu, Zhen-Lun Gu, Wen-Xuan Zhou, Ci-Yi Guo
Chun-Yu Liu, Department of Pharmacology, Medical College of Suzhou University, Suzhou 215007, Jiangsu Province, China
Zhen-Lun Gu, Suzhou Institute of Chinese Meteria Medica, Suzhou University, Suzhou 215007, Jiangsu Province, China
Wen-Xuan Zhou, Ci-Yi Guo, The Hong Kong Association for Health Care Ltd, Hong Kong, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Professor Zhen-Lun Gu, Suzhou Institute of Chinese Meteria Medica, Suzhou University, Suzhou 215007, Jiangsu Province, China.
Telephone: +86-512-65190599 Fax: +86-512-65190599
Received: August 16, 2004
Revised: October 2, 2004
Accepted: October 7, 2004
Published online: October 7, 2005

AIM: To observe the anti-liver fibrosis effect of Astragalus complanatus flavonoids (ACF) in rats.

METHODS: The liver fibrosis model in rats was established by injecting interperitoneally 0.2 mL/100 g 0.5% dimethylnitrosamine, thrice a week. Meanwhile, the rats were administered ACF (30, 60, 120 mg/kg) or colchicine (0.1 mg/kg) once a day for 1 mo. Serum N-propeptide of type I procollagen (PINP) and type III procollagen (PIIINP) was measured using ELISA. Malondialdehyde (MDA) and superoxide dismutase (SOD) in hepatic tissue were evaluated. Matrix metal protease-1 (MMP-1) mRNA expression was assayed by RT-PCR and the protein expression of tissue inhibitor of metal protease-1 (TIMP-1) was analyzed by immunohistochemistry.

RESULTS: In the ACF groups, SOD activity increased and MDA content decreased in comparison to the liver fibrosis model group. The serum PINP and PIIINP contents in ACF-2 and -3 group decreased compared to those in model group. In ACF-2 and -3 group, the expression of MMP-1 mRNA increased significantly and the protein expression of TIMP-1 decreased compared to that in model group.

CONCLUSION: The antifibrotic mechanisms of ACF are associated with its influence on lipid peroxidation and collagen synthesis and degradation.

Keywords: Astragalus complanatus, Liver fibrosis, N-propeptide of type I procollagen, N-propeptide of type III procollagen, Malondialdehyde, Superoxide dismutase, Matrix metal protease-1, Tissue inhibitor of metal protease-1