Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 21, 2005; 11(35): 5480-5484
Published online Sep 21, 2005. doi: 10.3748/wjg.v11.i35.5480
Effect of Jianweiyuyang granule on gastric ulcer recurrence and expression of VEGF mRNA in the healing process of gastric ulcer in rats
Xing-Ping Dai, Jia-Bang Li, Zhao-Qian Liu, Xiang Ding, Cheng-Hui Huang, Bing Zhou
Xing-Ping Dai, Jia-Bang Li, Cheng-Hui Huang, Bing Zhou, Institute of Integrated Traditional Chinese and Western Medicine, Central South University, Changsha 410078, Hunan Province, China
Zhao-Qian Liu, Institute of Clinical Pharmacology, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan Province, China
Xiang Ding, Department of Gastroenterology, Xiangya Hospital Central South University, Changsha 410078, Hunan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Foundation of Traditional Chinese Medicine of the Bureau of Health of Hunan Province, No. 202053
Correspondence to: Professor Jia-Bang Li, Institute of Integrated Traditional Chinese and Western Medicine, Central South University, Changsha 410078, Hunan Province, China. mwdlyy@sina.com
Telephone: +86-731-4327227
Received: November 23, 2004
Revised: January 9, 2005
Accepted: January 12, 2005
Published online: September 21, 2005
Abstract

AIM: To investigate the effect of Jianweiyuyang (JWYY) granule on gastric ulcer recurrence and its mechanism in the treatment of gastric ulcer in rats.

METHODS: Gastric ulcer in rats was induced according to Okeba’s method with minor modification and the recurrence model was induced by IL-1β. The expression of vascular endothelial growth factor mRNA (VEGF mRNA) was examined by reverse transcription polymerase chain reaction in gastric ulcer and microvessel density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry.

RESULTS: MVD was higher in the JWYY treatment group (14.0±2.62) compared with the normal, model and ranitidine treatment groups (2.2±0.84, 8.8±0.97, 10.4±0.97) in rats (P<0.01). The expression level of VEGF mRNA in gastric tissues during the healing process of JWYY treatment group rats significantly increased compared with other groups (normal group: 0.190±0.019, model group: 0.642±0.034, ranitidine group: 0.790±0.037, P<0.01).

CONCLUSION: JWYY granules can stimulate angiogenesis and enhance the expression of VEGF mRNA in gastric ulcer rats. This might be the mechanism for JWYY accelerating the ulcer healing, and preventing the recurrence of gastric ulcer.

Keywords: Jianweiyuyang granule, Gastric ulcer, VEGF mRNA