Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 7, 2005; 11(21): 3304-3306
Published online Jun 7, 2005. doi: 10.3748/wjg.v11.i21.3304
Mutation in D-loop region of mitochondrial DNA in gastric cancer and its significance
Yi-Bing Zhao, Hong-Yu Yang, Xi-Wei Zhang, Guo-Yu Chen
Yi-Bing Zhao, Hong-Yu Yang, Xi-Wei Zhang, Guo-Yu Chen, Department of Gastrointestinal Surgery, Jiangsu Provincial Hospital, Nanjing 210029, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Yi-Bing Zhao, Department of Gastrointestinal Surgery, Jiangsu Provincial Hospital, Nanjing 210029, Jiangsu Province, China. njzyb72@sina.com
Telephone: +86-25-83226889
Received: May 25, 2004
Revised: May 26, 2004
Accepted: June 24, 2004
Published online: June 7, 2005
Abstract

AIM: To investigate the mutation in D-loop region of mitoc-hondrial DNA in gastric cancer and its influence on the changes of reactive oxygen species (ROS) and cell cycle.

METHODS: The D-loop region was amplified by PCR and sequenced. Reactive oxygen species and cell cycle were detected by flow cytometry in 20 specimens from gastric cancer and adjacent normal tissues. According to the sequence results, gastric cancer tissue was divided into mutation group and control group. Reactive oxygen species, apoptosis and proliferation in the two groups were compared.

RESULTS: Among the 20 gastric cancer specimens, 18 mutations were identified in 7 patients, the mutation rate being 35%. There were four microsatellite instabilities in the mutations. No mutation was found in the adjacent tissues. Reactive oxygen species, apoptosis, and proliferation in the mutation group were all significantly higher than those in control group.

CONCLUSION: Mutation in D-loop region plays a role in the genesis and development of gastric cancer.

Keywords: Mitochondria, DNA, D-loop, Mutation, Reactive oxygen species