Brief Reports
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World J Gastroenterol. Mar 28, 2005; 11(12): 1809-1812
Published online Mar 28, 2005. doi: 10.3748/wjg.v11.i12.1809
Study on hepatoprotective effect of peptide S-8300 from shark liver
Feng-Jie Huang, Zheng-Bing Lv, Qian Li, Li-Jun Wei, Liang Zhang, Wu-Tong Wu
Feng-Jie Huang, Zheng-Bing Lv, Qian Li, Li-Jun Wei, Liang Zhang, Wu-Tong Wu, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Hi-Tech Research and Development Program of China (863 Program), No. 2001AA624090 and 2003AA624090; the National Natural Science Foundation of China, No. 30171103; Youth Foundation of China Pharmaceutical University, No. C0316
Correspondence to: Wu-Tong Wu, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China. wuwutongmailbox@163.com
Telephone: +86-25-83220372 Fax: +86-25-83220372
Received: September 8, 2004
Revised: September 9, 2004
Accepted: December 9, 2004
Published online: March 28, 2005
Abstract

AIM: To explore the effects of peptide S-8300 from shark liver (S-8300) on liver function as well as in regulating immune functions in experimental injury models.

METHODS: Mice were administered with different doses of S-8300 for four consecutive days, followed by mice injected with tetrachloromethane (CCl4) on d 3. The activity of ALT, AST, LDH, SOD and contents of MDA and GSH in the mice liver were tested. And mice were treated with Cy (100 mg/kg) at the beginning of the experiment to induce immunosuppression model. The S-8300 groups were treated with S-8300 seven days after the beginning of Cy administration. The effects of S-8300 on the formulation of serum hemolysin and the content of IL-2 in serum in the immunosuppression mice were observed.

RESULTS: S-8300 obviously decreased the level of ALT (52.2±11.0 U/dL vs 135.9±6.5 U/dL, P<0.01), AST (67.5±6.9 U/dL vs 238.8±8.7 U/dL, P<0.01), LDH (155.1±46.8 U/dL vs 240.4±6.0 U/dL, P<0.01) & MDA (0.64±0.027 nmol/mg vs 1.06±0.040 nmol/mg, P<0.01) and increased SOD (24.51±1.01 U/mg vs 19.05±0.73 U/mg, P<0.01) & GSH (24.17±0.91 µg/mg vs 14.93±0.45 µg/mg, P<0.01) in mice liver damaged by CCl4. S-8300 also markedly improved the formulation of serum hemolysin (0.094±0.005 A540vs 0.063±0.006 A540, P<0.01) and increased the level of IL-2 (9.74±1.16 pg/mL vs 5.81±0.87 pg/mL, P<0.01) in serum of the immunosuppression mice.

CONCLUSION: The results suggested S-8300 has significant hepatoprotective, immunomodulatory and inhibiting of lipid peroxidation activity.

Keywords: CCl4; Immunosuppression; IL-2; Hepatoprotective; Serum hemolysin