Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 1, 2004; 10(9): 1369-1371
Published online May 1, 2004. doi: 10.3748/wjg.v10.i9.1369
Diagnosis of intestinal acariasis with avidin-biotin system enzyme-linked immunosorbent assay
Rong-Bo Zhang, Yong Huang, Chao-Pin Li, Yu-Bao Cui
Rong-Bo Zhang, Chao-Pin Li, Yu-Bao Cui, Department of Etiology and Immunology, School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China
Yong Huang, Shandong Institute of Parasitic Diseases, Jining 272033, Shandong Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Chao-Pin Li, Department of Etiology and Immunology, School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China. cpli @aust.edu.cn
Telephone: +86-554-6658770 Fax: +86-554-6662469
Received: August 2, 2003
Revised: September 2, 2003
Accepted: September 18, 2003
Published online: May 1, 2004
Abstract

AIM: To explore the value of avidin-biotin system enzyme-linked immunosorbent assay (ABC-ELISA) in diagnosis of intestinal acariasis.

METHODS: Mite-specific IgG levels in serum of 48 patients with intestinal acariasis were measured with ABC-ELISA. The sensitivity of this method was compared with that of staphylococcal protein A enzyme-linked immunosorbent assay (SPA-ELISA).

RESULTS: The positive rate of mite-specific IgG detected with ABC-ELISA and SPA-ELISA was 89.58% (43/48) and 56.25% (27/48), respectively. The positive rate with ABC-ELISA was statistically higher than that with SPA-ELISA (χ2 = 13.50, P < 0.01).

CONCLUSION: ABC-ELISA is an effective method for the diagnosis of intestinal acariasis.

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