Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 1, 2004; 10(7): 1037-1042
Published online Apr 1, 2004. doi: 10.3748/wjg.v10.i7.1037
Ginkgo biloba extract reverses CCl4–induced liver fibrosis in rats
Yan-Jun Luo, Jie-Ping Yu, Zhao-Hong Shi, Li Wang
Yan-Jun Luo, Jie-Ping Yu, Department of Gastroenterology, Hubei Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China
Zhao-Hong Shi, Department of Gastroenterology, Wuhan First Hospital, Wuhan 430036, Hubei Province, China
Li Wang, Department of Geriatrics, Wuhan General Hospital, Guangzhou Command of PLA, Wuhan 430070, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Yan-Jun Luo, Department of Gastroenterology, Hubei Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
Telephone: +86-27-88058274
Received: August 23, 2003
Revised: September 17, 2003
Accepted: October 22, 2003
Published online: April 1, 2004

AIM: To study the reversing effect of Ginkgo biloba extract (GbE) on established liver fibrosis in rats.

METHODS: Following confirmation of CCl4-induced liver fibrosis, GbE or saline was administrated to the rats for 4 weeks. The remaining rats received neither CCl4 nor GbE as normal control. The four groups were compared in terms of serum enzymes, tissue damage, expression of αSMA and tissue inhibitor-1 of metalloproteinase (TIMP-1) and metalloproteinase-1 (MMP-1).

RESULTS: Compared with saline-treated group, liver fibrosis rats treated with GbE had decreased serum total bilirubin (P < 0.01) and aminotransferase levels (P < 0.01) and increased levels of serum albumin (P < 0.01). Microscopic studies revealed that the livers of rats receiving GbE showed allieviation in fibrosis (P < 0.05) as well as expression of αSMA (P<0.01). The liver collagen and reticulum contents were lower in rats treated with GbE than saline-treated group (P < 0.01). RT-PCR revealed that the level of TIMP-1 decreased while the level of MMP-1 increased in GbE group.

CONCLUSION: Administration of GbE improved CCl4–induced liver fibrosis. It is possibly attributed to its effect of inhibiting the expression of TIMP-1 and promoting the apoptosis of hepatic stellate cells.

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