Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 1, 2004; 10(21): 3194-3196
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3194
Gene expression profiling in Barrett’s esophagus and cardia intestinal metaplasia: A comparative analysis using cDNA microarray
Ying Chang, Jun Gong, Bin Liu, Jun Zhang, Fei Dai
Ying Chang, Jun Gong, Jun Zhang, Fei Dai, Department of Digestion, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Bin Liu, Department of Emergence Surgery, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Clinical Principal Discipline Foundation of Public Health Ministry of China, No.20012130
Correspondence to: Professor Jun Gong, Department of Digestion, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China. emulan@126.com
Telephone: +86-29-85212945 Fax: +86-29-87678758
Received: April 22, 2004
Revised: April 23, 2004
Accepted: April 29, 2004
Published online: November 1, 2004
Abstract

AIM: To study the difference of gene expression profile changes in Barrett’s esophagus (BE) and cardia intestinal metaplasia (CIM) and to screen the novel genes in the early stage by cDNA microarray.

METHODS: cDNA retrotranscribed from an equal amount of mRNA from BE and CIM epithelial tissues was labeled with Cy3 and Cy5 fluorescence as probes. The mixed probe was hybridized with three pieces of BiostarH-40 s double dot human whole gene chip. The chips were scanned with a ScanArray 4000. The acquired images were analyzed using GenePix Pro 3.0 software.

RESULTS: A total of 141 genes were screened out that exhibited different expression in all three chips. There were 74 upregulated and 67 downregulated genes in gene expression profiles of BE which were two times of that in CIM.

CONCLUSION: There is a difference in gene expression level between BE and CIM epithelia. These 141 genes probably relate to the occurrence and development of BE and the progression to adenocarcinoma.

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