Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 15, 2004; 10(2): 167-171
Published online Jan 15, 2004. doi: 10.3748/wjg.v10.i2.167
Inhibition of β-ionone on SGC-7901 cell proliferation and upregulation of metalloproteinases-1 and -2 expression
Jia-Ren Liu, Bao-Feng Yang, Bing-Qing Chen, Yan-Mei Yang, Hong-Wei Dong, You-Qiang Song
Jia-Ren Liu, Bing-Qing Chen, Yan-Mei Yang, Hong-Wei Dong, Public Health College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Bao-Feng Yang, Department of Pharmacology, Harbin Medical University, Harbin 150086, Heilongjiang Province, China You-Qiang Song, Department of Biochemistry and the Genome Research Centre, The University of Hong Kong, Hong Kong, China Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30200229 and the Youth Foundation of Harbin Medical University, China
Correspondence to: Dr. Jia-Ren Liu, Public Health College, Harbin Medical University, 199 Dongdazhi Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
Telephone: +86-451-3641309 Fax: +86-451-3648617
Received: March 4, 2003
Revised: March 23, 2003
Accepted: April 1, 2003
Published online: January 15, 2004

AIM: To observe the effect of β-ionone on the proliferation of human gastric adenocarcinoma cell line SGC-7901 and the inhibition of metalloproteinase.

METHODS: Using growth inhibition, Zymograms assays and reverse transcription-polymerase-chain reaction (RT-PCR), we examined cell growth rates, activities of matrix metalloproteinases-2 (MMP-2) and -9 (MMP-9), and expression of metalloproteinases-1 (TIMP-1) and -2 (TIMP-2) in SGC-7901 cells after the treatment with β-ionone for 24 h and 48 h, respectively.

RESULTS: β-ionone had an inhibitory effect on the growth of SGC-7901 cells. Eight days after the treatment with β-ionone at concentrations of 25, 50, 100 and 200 μmol/L, the inhibition rates were 25.9%, 28.2%, 74.4% and 90.1%, respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89 μmol/L. The effects of β-ionone on MMP-2 and MMP-9 activities in SGC-7901 cells were not observed. However, the levels of TIMP-1 and TIMP-2 transcripts were elevated in cells treated with β-ionone in a dose-dependent manner.

CONCLUSION: β-ionone can inhibit the proliferation of SGC-7901 cells, upregulate the expression of TIMP-1 and TIMP-2 expression, and may influence metastasis of cancer.

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