Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2560
Revised: October 4, 2003
Accepted: October 7, 2003
Published online: September 1, 2004
AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA.
METHODS: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test.
RESULTS: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.
CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.