Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 1, 2004; 10(11): 1619-1624
Published online Jun 1, 2004. doi: 10.3748/wjg.v10.i11.1619
Risk factors of development of gut-derived bacterial translocation in thermally injured rats
Zhong-Tang Wang, Yong-Ming Yao, Guang-Xia Xiao, Zhi-Yong Sheng
Zhong-Tang Wang, Yong-Ming Yao, Zhi-Yong Sheng, Department of Microbiology and Immunology, Burns Institute, 304th Hospital of PLA, Beijing 100037, China
Guang-Xia Xiao, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China
Author contributions: All authors contributed equally to the work.
Supported by the National Key Program for Fundamental Research and Development, No.G1999054203; the National Science Fund for Outstanding Young Scholars, No.30125020; the “10th Five-Year Plan” Scientific Research Foundation of Chinese PLA, No.01MA207
Correspondence to: Yong-Ming Yao, M.D., Department of Microbiology and Immunology, Burns Institute, 304th Hospital of PLA, 51 Fu-Cheng Road, Beijing 100037, China.
Telephone: +86-10-66867394 Fax: +86-10-68429998
Received: September 23, 2003
Revised: January 4, 2003
Accepted: December 29, 2003
Published online: June 1, 2004

AIM: Studies have demonstrated that gut-derived bacterial translocation (BT) might play a role in the occurrence of sepsis and multiple organ dysfunction syndrome (MODS). Yet, no convincing overall analysis of risk factors for BT has been reported. The purpose of this study was to evaluate the related factors for the development of BT in burned rats.

METHODS: Wistar rats were subjected to 30% third-degree burns. Then samples were taken on postburn d 1, 3, and 5. Incidence of BT and counts of mucosal bifidobacteria, fungi and E. coli, mucus sIgA, degree of injury to ileal mucosa, and plasma interleukin-6 were observed. Univariate analysis and multivariate logistic regression analysis were performed.

RESULTS: The overall BT rate was 53.9% (69 in 128). The result of univariate analysis showed that the levels of plasma endotoxin and interleukin-6, the counts of mucosal fungi and E. coli, and the scores of ileum lesion were markedly increased in animals with BT compared with those without (P = 0.000-0.005), while the levels of mucus sIgA and the counts of mucosal bifidobacteria were significantly reduced in animals with translocation compared with those without (P = 0.000). There was a significant positive correlation between mucus sIgA and the counts of mucosal bifidobacteria (r = 0.74, P = 0.001). Moreover, there were strong negative correlations between scores of ileum-lesion and counts of bifidobacteria (r = -0.67, P = 0.001). Multivariate logistic regression revealed that ileum lesion score (odds ratio [OR] 45.52, 95% confidence interval [CI] 5.25-394.80), and counts of mucosal bifidobacteria (OR 0.039, 95% CI 0.0032-0.48) were independent predictors of BT secondary to severe burns.

CONCLUSION: Ileal lesion score and counts of mucosal bifidobacteria can be chosen as independent prognosis factors of the development of BT. Specific interventions targeting these high-risk factors might be implemented to attenuate BT, including strategies for repair of damaged intestinal mucosae and restoration of the balance of gastrointestinal flora.

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